Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Ameliorative effect of Citrus aurantifolia and Cinnamomum burmannii extracts on diabetic complications in a hyperglycemic rat model

Herin Mawarti , Khotimah , Mohammad Zulfikar As'ad, Mukhammad Rajin

Department of Nursing Sciences, Faculty of Health Sciences, Pesantren Tinggi Darul Ulum University, Jombang, East Java 61481, Indonesia;

For correspondence:- Herin Mawarti Email: herinmawarti@fik.unipdu.ac.id Tel:+6285736347827

Accepted: 18 April 2018 Published: 28 May 2018

Citation: Mawarti H, K, As'ad MZ, Rajin M. Ameliorative effect of Citrus aurantifolia and Cinnamomum burmannii extracts on diabetic complications in a hyperglycemic rat model. Trop J Pharm Res 2018; 17(5):823-829 doi: 10.4314/tjpr.v17i5.11

© 2018 The authors.
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Abstract

Purpose: To evaluate the effects of Citrus aurantifolia and Cinnamomum burmannii extracts (Jermanis) on the number of pancreatic β-cells and levels of superoxide dismutase (SOD), low-density lipoprotein (LDL), and transforming growth factor-beta (TGF-β) in β-cells.

Methods: Various doses of a combination of extracts of C. aurantifolia and C. burmannii were given orally to 25 male Wistar rats (Rattus norvegicus) every 2 h for 30 days. Doses of 100, 300 and 500 mg/kg/day C. aurantifolia extract were considered low, medium and high doses, respectively, while, 200, 400 and 800 mg/kg/day C. burmannii extract were considered low, medium and high doses, respectively. LDL and SOD levels in blood serum were analyzed spectrophotometrically. TGF-β expression was evaluated by immunohistochemistry. Pancreatic tissue sections (diameter of a β-cell) were evaluated by light microscopy after hematoxylin and eosin staining.

Results: The combination of C. aurantifolia and C. burmannii extracts increased SOD levels, TGF-β expression and the number of β-cells and decreased LDL levels in hyperglycemic rats. The results indicate that the highest doses of C. aurantifolia and C. burmannii increased the number of β-cells in the islets of Langerhans. The combined extracts of C. aurantifolia and C. burmannii significantly affected pancreatic cell regeneration.

Conclusion: The combination of C. aurantifolia and C. burmannii extracts may be a promising alternative preventative medicine for management of diabetic complications in patients with hyperglycemia.

Keywords: ^6;-cell, Diabetes, Diabetic complications, Citrus aurantifolia, Cinnamomum burmannii, Biochemical profile, TGF-^6; expression