Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antibacterial potential of extracts of various parts of Catunaregam tomentosa (Blume ex DC) Tirveng and their effects on bacterial granularity and membrane integrity

Karnsiree Thimabut¹, Amornrat Keawkumpai¹, Patima Permpoonpattana¹, Saranyoo Klaiklay², Parinuch Chumkaew², Darika Kongrit¹, Jaraslak Pechwang¹, Theera Srisawat¹

¹Department of Agricultural Science and Technology; ²Department of Applied Chemistry, Faculty of Science and Industrial Technology, Prince of Songkla University, Suratthani Campus, 31 Moo 6 Thumbol Makhamtia, Muang, Suratthani 84000, Thailand.

For correspondence:- Theera Srisawat Email: theera.s@psu.ac.th Tel:+6677355453

Accepted: 18 April 2018 Published: 28 May 2018

Citation: Thimabut K, Keawkumpai A, Permpoonpattana P, Klaiklay S, Chumkaew P, Kongrit D, et al. Antibacterial potential of extracts of various parts of Catunaregam tomentosa (Blume ex DC) Tirveng and their effects on bacterial granularity and membrane integrity. Trop J Pharm Res 2018; 17(5):875-882 doi: 10.4314/tjpr.v17i5.18

© 2018 The authors.
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Abstract

Purpose: To investigate the antibacterial activity of extracts from Catunaregam tomentosa on Bacillus subtilis and Staphylococcus aureus, and the bacterial responses to the extracts.

Methods: The antibacterial activity of fruit, leaf and stem bark extracts were evaluated against B. subtilis (ATCC6633) and S. aureus (ATCC25923). Using a disc diffusion method, extracts at concentrations ranging from 50 – 1,000 µg/disc were tested. The minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of the extracts against the test bacteria were determined. Fluorescent activated cell sorting (FACS) was used to assess the responses of both types of bacteria to the extracts.

Results: The fruit and leaf extracts at 1,000 µg/disc showed optimum efficacy against B. subtilis and S. aureus with MIC of 1,000 µg/mL against both B. subtilis and S. aureus, for the fruit and the leaf extracts. With increasing doses of fruit and leaf extracts at 6 h of incubation, FACS profiles revealed that cell death for B. subtilis increased. The fruit and leaf extracts of C. tomentosa also exhibited antibacterial activity against S. aureus in a dose- and time-dependent manner. The bacteria initially lost their granularity, then lost membrane integrity, and consequently died.

Conclusion: The fruit and leaf extracts of C. tomentosa exhibit significant antibacterial potential against Gram-positive bacteria by damaging bacterial granularity and membrane integrity.

Keywords: Catunaregam tomentosa, Flow cytometry, Programmed cell death, Response pattern, Bacterial granularity, Membrane integrity