Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Phytochemical profile, antioxidant, antimicrobial and antipancreatic lipase activities of fermented Camellia japonica L leaf extracts

Seo Hyun Moon¹, Min Young Kim²

¹Department of Forensic DNA, National Forensic Service, Wonju, Gangwon-do; ²Toxicology Laboratory, Faculty of Biotechnology (Biomaterials), College of Applied Life Science, SARI, Jeju National University, Jeju, Republic of Korea.

For correspondence:- Min Kim Email: jeffmkim@jejunu.ac.kr Tel:+82647543349

Accepted: 23 April 2018 Published: 28 May 2018

Citation: Moon SH, Kim MY. Phytochemical profile, antioxidant, antimicrobial and antipancreatic lipase activities of fermented Camellia japonica L leaf extracts. Trop J Pharm Res 2018; 17(5):905-912 doi: 10.4314/tjpr.v17i5.22

© 2018 The authors.
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Abstract

Purpose: To investigate the probable antioxidant, antimicrobial and antipancreatic lipase effects of fermented Camellia japonica leaf extracts.

Methods: Camellia japonica leaves fermented with Nuruk were extracted using methanol and ethanol. Total phenolic, flavonoid, carotenoid and L-ascorbic acid contents were determined by UV-visible spectrophotometry. The antioxidant activities of these extracts were determined by free radical scavenging, ferrous ion chelating and reducing power assays. Their antimicrobial properties against Gram-positive Staphylococcus epidermidis and Bacillus subtilis, and Gram-negative Klebsiella pneumonia and Escherichia coli bacteria were evaluated by disc diffusion method. Inhibition of pancreatic lipase was measured based on the hydrolytic reaction of p-nitrophenyl butyrate with pancreatic lipase.

Results: The ethanol extracts of fermented Camellia japonica leaves exhibited higher phenolic (32274 mg GAE/100 g) and flavonoid (20519 mg RE/100 g) contents with higher superoxide (IC₅₀ = 0.23 mg/mL), hydrogen peroxide (IC₅₀ = 0.28 mg/mL) radical scavenging and ferrous ion chelating (IC₅₀ = 0.21 mg/mL) activities than those of methanol. These ethanol extracts also showed higher antimicrobial activities against all bacterial strains tested with higher inhibitory effects on pancreatic lipase than methanol extracts.

Conclusion: The results highlight the possible use of fermented Camellia japonica leaf extracts as a source of antioxidant, antibacterial and antiobesity agents. Ethanol is recommended as solvent for extracting antioxidants, antibacterial and antiobesity agents from this plant

Keywords: Antioxidant activity, Antimicrobial activity, Fermented Camellia japonica extracts, Pancreatic lipase inhibition