Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of biochemical constituents and inhibitory effect of tea clone 100 on colorectal cancer cell line HCT-116

Fereydoon Bondarian¹, Asa Ebrahimi² , Frouzandeh Mahjoubi³, Eslam Majidi Hervan¹, Azadi Gonbad⁴

¹Department of Plant Breeding; ²Department of Biotechnology, Science and Research branch, Islamic Azad University, Tehran; ³Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology, Karaj; ⁴Department of Seed and Plant Improvement, Tea Research Institute (TRI), Guilan 44159-77555, Iran.

For correspondence:- Asa Ebrahimi Email: dr.asaebrahimi@gmail.com Tel:+989128057492

Accepted: 9 May 2018 Published: 30 June 2018

Citation: Bondarian F, Ebrahimi A, Mahjoubi F, Hervan EM, Gonbad A. Evaluation of biochemical constituents and inhibitory effect of tea clone 100 on colorectal cancer cell line HCT-116. Trop J Pharm Res 2018; 17(6):1033-1041 doi: 10.4314/tjpr.v17i6.8

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the total content of polyphenols and the free radical scavenging activity of three different extracts of three types of tea clone 100 (black, green and white), and their anti-proliferative effects on colorectal cancer.

Methods: Five major polyphenols, viz, (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate and (-)-epigallocatechin gallate, were identified using thin layer chromatography (TLC). Catechins were quantified by high performance liquid chromatography (HPLC). Antioxidant activity was measured by DPPH radical scavenging method, while 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was employed for the determination of cell viability of colon cell line HCT-116 after 24 and 48 h.

Results: The aqueous methanol (70 %) extract of white tea yielded the highest amount of polyphenols (36.67 ± 0.54 mg GAE/g dry weight). The DPPH radical scavenging activity of white tea was 71.74 ± 0.42 %, and it produced high anti-proliferation activity against colorectal cancer cell line HCT-116 (86.06 ± 0.54 %).

Conclusion: White tea extract possesses high DPPH radical scavenging activity, and exerts good anti-proliferative effects against colorectal cancer cell line HCT-116, most likely due to its catechin content.

Keywords: Camellia sinensis L., Catechin, DPPH radical scavenging, Anti-cancer