Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of ankaferd blood stopper on early bone tissue healing in extraction sockets: an experimental in vivo study

Hatice Balci Yuce¹, Nihat Akbulut² , Sibel Akbulut³, Kemal Özgür Demiralp⁴, Zafer Karaca⁵, Kaan Orhan⁶

¹Department of Periodontology; ²Department of Oral and Maxillofacial Surgery; ³Department of Orthodontics, Faculty of Dentistry, Gaziosmanpa#1;a University, Tokat; ⁴Ministry of Health Public Hospitals Agency of Turkey, Ankara; ⁵Department of Histology, Faculty of Medicine, Gaziosmanpa#1;a University, Tokat; ⁶Dentomaxillofacial Radiology Department, Dentistry Faculty, Ankara University, Ankara, Turkey.

For correspondence:- Nihat Akbulut Email: drnihatakbulut@yahoo.com Tel:+905054489263

Received: 19 January 2015 Accepted: 29 June 2015 Published: 30 August 2015

Citation: Yuce HB, Akbulut N, Akbulut S, Demiralp K&, Karaca Z, Orhan K. Effect of ankaferd blood stopper on early bone tissue healing in extraction sockets: an experimental in vivo study. Trop J Pharm Res 2015; 14(8):1469-1473 doi: 10.4314/tjpr.v14i8.20

© 2015 The authors.
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Abstract

Purpose: To investigate the effect of Ankaferd blood stopper (ABS) on early bone healing of extraction sockets in rats.

Methods: Twenty-eight male Wistar rats were divided into four groups. The maxillary right first molar tooth of the rats was extracted under general anesthesia. Two groups (C-1, n = 6 and C-2, n = 6) received saline solutions in the extraction sockets immediately and one day after the extraction, respectively, while two groups (A-1, n = 8 and A-2, n = 8) received ABS. The rats in A-1 and C-1 groups were sacrificed after 7 days of post-extraction while the rats in A-2 and C-2 groups were sacrificed after 28 days. Bone samples were taken from the maxillas, and tissues were prepared for histopathological analysis. Osteoid tissue (OT), mineralized bone tissue (MT), remaining area (RA), and inflammatory cell infiltration (ICI) were determined. The histomorphometric results were analyzed statistically using analysis of variance (ANOVA).

Results: Osteoid formation was highest in C-1 group (39.71 ± 9.68, p < 0.05). Differences in OT among other groups were not significant (p > 0.05). MT was higher in C-2 group (47.73 ± 12.15) than in other groups (p < 0.05). RA was highest in C-1 group (59.95 ± 12.75). ICI was significantly lower in control (C1 and C-2) groups than in ABS (A-1 and A-2) groups (p < 0.05).

Conclusion: ABS administered topically to extraction sockets immediately after extraction has no effect on bone healing; in fact, ABS increases inflammation in vivo.

Keywords: Ankaferd blood stopper, Wound healing, Mineralized bone tissue, Inflammatory cell infiltration, Osteoid tissue, Tooth extraction