Fen Wen1,
Yuxian Lu2,
Ke Xu3,
Yanmei Liu3,
Xiaojuan Qian4,
Dezhi Bian3 
For correspondence:- Dezhi Bian Email: dezhiBian2008@163.com
Accepted: 20 June 2018 Published: 28 July 2018
Citation: Wen F, Lu Y, Xu K, Liu Y, Qian X, Bian D. Diosgenin inhibits cell proliferation of primary human thyrocytes via downregulation of PI3K/Akt signaling pathway. Trop J Pharm Res 2018; 17(7):1243-1248 doi: 10.4314/tjpr.v17i7.3
© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To determine the potential influence of diosgenin on proliferation of human thyrocytes and its possible mechanism.
Methods: Primary human thyrocytes were cultured and treated with diosgenin at various time intervals. Anti-proliferative activity was determined by MTT assay. Cell proliferation was evaluated by EdU assay while cell cycle was analyzed using fluorescence-activated cell sorting (FACS) method. Protein ex
Results: Diosgenin inhibited proliferation of primary human thyrocytes and caused G0/G1 arrest in a concentration-dependent manner. It also downregulated cyclin D1 and phosphorylation of PI3K and Akt, but upregulated p21 and p27.
Conclusion: Inhibition of proliferation of primary human thyrocytes by diosgenin occurs via downregulation of PI3K/Akt signaling pathway. Therefore, diosgenin can be developed as a potential drug for the treatment of thyroid disease.
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