Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Suppression of long non-coding RNA H19 inhibits proliferation, cell migration and invasion in human cervical cancer cells

Huawei Xin¹, Mingzhe Li², Xiaoling Cheng¹, Tao Wang², Xiaoliu Liu³, Yan Zhang⁴

¹College of Life Science and HealthA292;Wuhan University of Science and Technology; ²Medical School, Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Wuhan University of Science and Technology, Wu Han 430065; ⁴Department of Gynaecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan 430060, China.

For correspondence:- Yan Zhang Email: IzettaNnna@yahoo.com

Accepted: 19 June 2018 Published: 28 July 2018

Citation: Xin H, Li M, Cheng X, Wang T, Liu X, Zhang Y. Suppression of long non-coding RNA H19 inhibits proliferation, cell migration and invasion in human cervical cancer cells. Trop J Pharm Res 2018; 17(7):1249-1253 doi: 10.4314/tjpr.v17i7.4

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the expression profile of lncRNA H19 in different cervical cancers, and to decipher its function in the growth and metastasis of cervical cancer.

Methods: The analysis LncRNA H19 expression was performed using quantitative real time polymerase chain reaction (qRT-PCR). Cell counting kit 8 (CCK8) assay was used to assess the viability of the cells. The cells were transfected with Si-H19 using Lipofectamine 2000 and the metastasis of cells was determined by cell migration and invasion assay. Immunoblotting was used to evaluate the protein expression.

Results: The lncRNA H19 expression was considerably enhanced in cervical cancer cells, and was about 2.6 to 5.3 times more in cervical cancer cells relative to non-cancer cells. Inhibition of lncRNA caused significant reduction in cervical cancer cell growth in a time-dependent manner. In addition while silencing of lncRNA inhibited the metastasis of HeLa cells. Cell migration and invasion was about 26 % in Si-H19 transfected cervical cancer cells, relative to 65 % in Si-NC cervical HeLa cells. Similarly, cell invasion was 45 % in Si-H19 cervical HeLa cells relative to the negative control (Si-NC). Inhibition of HeLa cell metastasis was also concomitant with decline of metalloproteinases (MMP)-2 and 9 expression.

Conclusion: lncRNA regulates the growth and metastasis of cervical cancer cells. Thus, IncRNA may be an important therapeutic agent for cervical cancer.

Keywords: Cervical cancer, lncRNA, Proliferation, Invasion