Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of maternal diabetes and quercetin exposure on the oxidative stress and kidney damage in rat fetus

Saeedeh Heidarinazhad¹, Mahmood Khaksary Mahabady¹ , Reza Ranjbar¹, Hossein Najafzadeh Varzi^2,3, Babak Mohammadian⁴, Mohammad Reza Tabandeh⁵

¹Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran; ²Department of Pharmacology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz; ³Molecular Biology Research Center, Health Research Institute, Babol University Medical Sciences, Babol; ⁴Department of Pathology; ⁵Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

For correspondence:- Mahmood Mahabady Email: mkhaksary@scu.ac.ir Tel:+989131619252

Accepted: 20 June 2018 Published: 28 July 2018

Citation: Heidarinazhad S, Mahabady MK, Ranjbar R, Varzi HN, Mohammadian B, Tabandeh MR. Effect of maternal diabetes and quercetin exposure on the oxidative stress and kidney damage in rat fetus. Trop J Pharm Res 2018; 17(7):1325-1330 doi: 10.4314/tjpr.v17i7.14

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the prophylactic effects of quercetin on oxidative stress and damage to kidney in rat's fetus harvested from diabetic mothers.

Methods: Female Wistar rats were divided to into four experimental groups: control, quercetin, diabetic, and quercetin-treated diabetic groups (6 rats in each group). Experimental diabetes was induced by intravenous injection of streptozotocin (50 mg/kg) and the female rats were mated with male rats. Thereafter, quercetin was orally administered by oral gavage (75 mg/kg), on 0, 7, 14 and 20 days of gestation. Foetuses were harvested on the 20th day of gestation and their kidneys, removed and the tissues examined biochemically and histopathologically. Subsequently, malondialdehyde (MDA) level, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were measured in the renal tissue.

Results: Maternal diabetes delayed fetal kidney development and renal tubular necrosis, and reduced the number of renal glomeruli, while quercetin decreased the level of these changes. Accordingly, the MDA level increased while catalase, superoxide dismutase and glutathione peroxidase activities decreased in diabetic fetal kidney. These biochemical changes were corrected by quercetin.

Conclusion: Quercetin has a protective effect on some biochemical and pathological changes in the kidney of foetuses exposed to maternal diabetes.

Keywords: Maternal diabetes, Streptozotocin, Quercetin, Renal teratogenicity, Fetus