Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Indonesian Pasuruan propolis extract does not exert anti-proliferation and pro-apoptotic effect on human colon carcinoma cell line HT-29

Ihda Dian Kusuma^1,2 , Aris Rosidah^1,3, Eviana Norahmawati², Nurdiana ³, Agustina Tri Endharti⁵

¹Biomedical Magister Program, Faculty of Medicine, Universitas Brawijaya; ²Department of Pathology Anatomy, Faculty of Medicine, Universitas Brawijaya; ³Department of Pharmacology, Faculty of Medicine, Islamic University of Malang; ⁴Department of Pharmacology, Faculty of Medicine, Universitas Brawijaya; ⁵Department of Parasitology, Faculty of Medicine, Universitas Brawijaya, Indonesia.

For correspondence:- Ihda Kusuma Email: ihdadiankusuma@ymail.com

Accepted: 18 June 2018 Published: 28 July 2018

Citation: Kusuma ID, Rosidah A, Norahmawati E, N, Endharti AT. Indonesian Pasuruan propolis extract does not exert anti-proliferation and pro-apoptotic effect on human colon carcinoma cell line HT-29. Trop J Pharm Res 2018; 17(7):1345-1351 doi: 10.4314/tjpr.v17i7.17

© 2018 The authors.
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Abstract

Purpose: To evaluate the anti-cancer activity of Indonesian Pasuruan propolis extract (PPE) against HT-29 human colon carcinoma cell line.

Methods: HT-29 cells were cultured and treated in different concentrations of PPE (50, 100, 200, 400 µg/mL) for 24 h. The cells were evaluated by several indicators such as cell proliferation and apoptosis as well as the expression of protein Ki67, p53, cyclin D1, and Bcl-xL.

Results: Administration of PPE inhibited cell proliferation of HT-29 in concentration-dependent manner but the effect was not significant (p = 0.842); the results were similar with regard to the expression of Ki67 in HT-29 cells (p = 0.953). Administration of 400 µg/mL PPE insignificantly decreased cyclin D1 expression (p = 0.149). The concentration of PPE at 50, 100, and 200 µg/mL induced cell apoptosis of HT-29 cells initially, but the level of apoptosis subsequently decreased (p = 0.416). Furthermore, the expressions of p53 and Bcl-xL decreased following treatment with PPE at 50, 100 and 200 µg/mL but increased for the PPE 400 µg/mL group (p = 0.000).

Conclusion: PPE reduced the expressions of p53, Ki67, cyclin D1, and Bcl-xL insignificantly as it it generally failed inhibit cell proliferation and promote cell apoptosis of HT-29 cells.

Keywords: Bcl-xL, Colon cancer, Cyclin D1, Human colon adenocarcinoma cell line HT-29, Proliferation marker Ki67, Tumor protein p53