Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MiR-145 inhibits proliferation of primary colon adenocarcinoma cells via induction of apoptosis, cell cycle arrest and inhibition of cell migration

Yong Yang¹, Peng Li², Xu-feng Ding², Xi Ming², Xiu-tian Guo¹

¹Department of Anus and Intestine; ²College of Clinical Medicine, Shanghai Hospital of Traditional Chinese Medicine, Shanghai 200071, China.

For correspondence:- Xiu-tian Guo Email: GastInellazalo@yahoo.com Tel:+862156639828

Accepted: 22 July 2018 Published: 31 August 2018

Citation: Yang Y, Li P, Ding X, Ming X, Guo X. MiR-145 inhibits proliferation of primary colon adenocarcinoma cells via induction of apoptosis, cell cycle arrest and inhibition of cell migration. Trop J Pharm Res 2018; 17(8):1491-1495 doi: 10.4314/tjpr.v17i8.5

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the role and potential of miR-145 as a therapeutic target for the treatment of primary colon adenocarcinoma in cell lines

Methods: The expression of miR145 was determined by quantitative real time-polymerase chain reaction (RT-PCR), while cell viability was determined by MTT assay. Apoptosis was assessed by 4',6-diamidino-2-phenylindole (DAPI), acridine orange/ethidium bromide (AO/EB), and annexin V/PI double staining. Cell cycle analysis was performed by flow cytometry, while immunoblotting was used to determine protein expression.

Results: The expression of miR-145 was significantly enhanced in all the colon adenocarcinoma cell lines investigated. On the other hand, suppression of miR-145 expression led to significant decrease in cell viability, activation of apoptosis, G2/M cell cycle arrest, and inhibition of migration of colon adenocarcinoma cells.

Conclusion: These results indicate that miR-145 regulates the proliferation and metastasis of colon adenocarcinoma cells. Thus, it may be a prospective drug target for the treatment of this disease

Keywords: MicroRNA, Apoptosis, Cell migration, Proliferation