Amna Khalid1,
Sammia Shahid1,
Shakeel Ahmad Khan1 
,
Sadia Kanwal2,
Atif Yaqoob3,
Zahid Ghulam Rasool1,
Komal Rizwan4
For correspondence:- Shakeel Khan Email: Shakilahmad56@gmail.com Tel:+82106638912
Accepted: 25 July 2018 Published: 31 August 2018
Citation: Khalid A, Shahid S, Khan SA, Kanwal S, Yaqoob A, Rasool ZG, et al. Antioxidant activity and hepatoprotective effect of Cichorium intybus (Kasni) seed extract against carbon tetrachloride-induced liver toxicity in rats. Trop J Pharm Res 2018; 17(8):1531-1538 doi: 10.4314/tjpr.v17i8.10
© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To assess the antioxidant and hepatoprotective activity of the aqueous-methanol extract of Cichorium intybus seeds (C. intybus) against carbon tetrachloride (CCl4)-induced liver toxicity in albino Wistar rats.
Method: The seed extract of C. intybus was prepared in aqueous methanol (20:80) via Soxhlet solvent extraction process. CCl4 (0.8 mL/kg) was administered to induce hepatic damage in Wistar rats. The seed extract (100, 250 and 500 mg/kg doses) and a 25 mg/kg dose of silymarin (as standard drug) were administered orally to separate groups of albino Wistar rats for 14 days. Blood samples from the rats were analyzed for biochemical markers for hepatic injury. The tissue samples of the rats were subjected to histopathological studies as well as analyzed for liver antioxidants.
Results: The results for biochemical markers revealed that the rats treated with the extract (500 mg/kg dose) showed a maximum elevation of catalase (48.90 µmole of H2O2 consumed/min/mg protein), glutothione peroxidase (22.1 mg GSH consumed/min/mg protein), superoxide dismutase (14.2 units/min/mg protein), and a reduction in glutathione (18.1 µmole of GSH/mg protein). Serum biochemical parameters including serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphate (ALKP), and direct bilirubin were significantly (p < 0.01) increased in the treated groups. Oral administration of different doses of C. intybus seed extract significantly (p < 0.01) protected the hepatic cells from impairment. The biochemical markers and hematological parameters were also normal in extract-treated rats in contrast to the standard (silymarin) and control groups.
Conclusion: The results show that C. intybus plant is potential a good natural source of natural hepatoprotective and antioxidants agents.
Archives
News Updates
Fulltext in PDF