Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Toxicity evaluation of standardized and nanoliposomal extracts of Labisia pumila whole plant (Blume, Myrsinaceae) in Sprague Dawley rats

Mohammed Ali Ahmed Saeed¹, Abdul Hakeem Memon², Mohd Shahrul Ridzuan Hamil¹, Hooi Kheng Beh³, Sultan Ayesh Mohammed Saghir⁴, Gurjeet Kaur⁵, Amirin Sadikun¹, Zhari Ismail¹

¹Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia; ²Department of Pharmacognosy, Faculty of Pharmacy, University of Sindh, Jamashoro, Pakistan; ³Faculty of Agro Based Industry, Universiti Malaysia Kelantan, 16100 Kota Bharu, Kelantan, Malaysia; ⁴Department of Pharmacology, School of Pharmaceutical Sciences; ⁵Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Pulau Pinang, Malaysia.

For correspondence:- Zhari Ismail Email: ismailzhari@gmail.com

Accepted: 17 July 2018 Published: 31 August 2018

Citation: Saeed MA, Memon AH, Hamil MS, Beh HK, Saghir SA, Kaur G, et al. Toxicity evaluation of standardized and nanoliposomal extracts of Labisia pumila whole plant (Blume, Myrsinaceae) in Sprague Dawley rats. Trop J Pharm Res 2018; 17(8):1557-1564 doi: 10.4314/tjpr.v17i8.13

© 2018 The authors.
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Abstract

Purpose: To investigate the toxicity of Labisia pumila standardized extract (LPE) and its liposomal extract (LLP).

Methods: For acute toxicity study, LPE or LLP was orally administered (2000 mg/kg) in single doses to Sprague Dawley rats and the routine activity of the rats was continuously monitored for a total of 14 days. After 14 days of treatment, all rats were sacrificed and their vital organs were excised, weighed and macroscopically examined, while for a repeated dose toxicity study, the rats were orally administered with LPE or LLP at the selected doses (250, 500 and 1000 mg/kg) for a period of 28 days. The animals were sacrificed (anaesthetized by sodium pentobarbitone and blood was collected by cardiac puncture), followed by examination of their body organs and blood serum.

Results: LPE and LLP at 2000 mg/kg did not produce mortality or significant changes in the general behaviour, body weight and organ gross appearance of the rats. In repeated dose toxicity study no significant changes in, growth, organ weights, haematological parameters, biochemical values and histological features of vital organs of the treated groups, compared to the control group.

Conclusion: The no-adverse-effect-level for LPE and LLP is (1000 mg/kg/day) when administered orally for 28 days.

Keywords: Labisia pumila, Acute oral toxicity, Repeated dose toxicity