Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Evaluation of protective effect of cyclodextrin glucanotransferase-treated Gastrodia elata Blume extract on ultraviolet B-induced premature skin aging

Eunson Hwang¹, Hien TT Ngo², Jung-Eun Yang¹, Sang-Yong Park¹, Jahyun Bae¹, Tae-Hoo Yi²

¹SD Biotechnologies Co. Ltd, #301 Seoul Hightech Venture Center, 29, Gonghang-daero 61-gil, Ganseo-gu, Seoul, 07563; ²Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.

For correspondence:- Tae-Hoo Yi Email: drhoo@khu.ac.kr Tel:+82312013693

Accepted: 27 September 2018 Published: 31 October 2018

Citation: Hwang E, Ngo HT, Yang J, Park S, Bae J, Yi T. Evaluation of protective effect of cyclodextrin glucanotransferase-treated Gastrodia elata Blume extract on ultraviolet B-induced premature skin aging. Trop J Pharm Res 2018; 17(10):1969-1975 doi: 10.4314/tjpr.v17i10.11

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the protective effect of Gastrodia elata Blume (G. elata, GE) and cyclodextrin glucanotransferase (CGTase) enzyme-treated G. elata extract (EGE) against premature skin aging using ultraviolet B (UVB)-exposed normal human dermal fibroblasts (NHDFs).

Methods: The extract was characterized by liquid chromatography with tandem mass spectrometry (LC-MS/MS), ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC–QToF–MS) and nuclear magnetic resonance spectroscopy (NMR). The expression of matrix metalloproteinases (MMP-1,3), interleukin-6 (IL-6), transforming growth factor (TGF-β1) and procollagen type I was assayed using ELISA kits. Safety evaluation of EGE’s dietary administration and topical application was performed by in vivo acute oral toxicity and local lymph node tests.

Results: Lower MMP-1 and IL-6 and higher procollagen type I and TGF-β1 levels were observed after treatment with EGE than with GE, indicating that EGE was more effective than GE in treating UVB-induced photoaging. With respect to phenolic composition, EGE had lower 4-hydroxybenzaldehyde (4-HBA) level and higher α-gastrodin level than GE. In UVB-irradiated NHDFs, α-gastrodin exhibited higher anti-aging activity than 4-HBA and β-gastrodin based on the expression of MMP-1, MMP-3, and procollagen type I. The in vivo data indicate that EGE was safe at concentrations of up to 2000 mg/kg for dietary administration and 0.1 % for topical application.

Conclusion: EGE protects UVB-induced photoaged human skin better than GE owing to its higher α-gastrodin content. Thus, EGE may be potentially useful agent in anti-aging cosmetic products.

Keywords: Gastrodia elata, ^5;-Gastrodin, Anti-aging, CGTase, Ultraviolet B (UVB) irradiation, Matrix metalloproteinase, Procollagen, Normal human dermal fibrob