Sivakumar Sivagurunathan Moni1 
,
Mohammad Firoz Alam2,
Hafiz A Makeen3,
Aamena Jabeen1,
Syeda Sanobar4,
Rahimullah Siddiqui2,
Remesh Moochikkal5,
Soliman Fouda1
For correspondence:- Sivakumar Moni Email: drsmsivakumar@gmail.com
Accepted: 28 October 2018 Published: 30 November 2018
Citation: Moni SS, Alam MF, Makeen HA, Jabeen A, Sanobar S, Siddiqui R, et al. Therapeutic potential of oleic acid nanovesicles prepared from petroleum ether extract of Sargassum binderi in streptozotocin-induced diabetic wound in Wistar rats. Trop J Pharm Res 2018; 17(11):2123-2128 doi: 10.4314/tjpr.v17i11.2
© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To study the effectiveness of phyto-oleic acid nanovesicles (PONVs) developed from Sargassum binderi (an alga) in healing diabetic wound in a rat model, and to establish the associated changes in cytokine network.
Methods: Phyto-extract was obtained from the whole plant of Sargassum binderi by Soxhlet extraction using petroleum ether as solvent. The crude extract was subjected to phytochemical analysis and used in the formulation of POVNs. The PONVs were formulated by entrapping petroleum ether extract of Sargassum binderi using the film hydration technique. Wound healing property was determined by measuring both pro-inflammatory and anti-inflammatory cytokines using enzyme-linked immunosorbent assay (ELISA).
Results: Tannins and steroids were the major components of the petroleum ether extract of Sargassum binderi. Serum cytokine levels were increased after inducing diabetes and creating the wound. The serum levels of IL-2, TNF-α and IL-1β were 37.3 ± 3.3, 76.3 ± 5.2 and 3307.6 ± 350 pg/ml, respectively. Treatment with PONVs modulated the serum cytokine levels through significant decreases in serum IL-2, TNF-α, IL-1β levels, and significant elevation of serum IL-4.
Conclusion: These results indicate that PONVs have promising potentials for application as topical treatment for diabetic wounds.
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