Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Cardioprotective effect of salvianolic acid B against isoproterenol-induced inflammation and histological changes in a cardiotoxicity rat model

Jun Liu, Liang Chen, Huihe Lu

Department of Cardiovascular Medicine, The First Hospital of Nantong, Nantong 226000, China;

For correspondence:- Huihe Lu Email: TheresahThompsonja@yahoo.com Tel:+8651385061272

Accepted: 26 August 2018 Published: 01 December 2018

Citation: Liu J, Chen L, Lu H. Cardioprotective effect of salvianolic acid B against isoproterenol-induced inflammation and histological changes in a cardiotoxicity rat model. Trop J Pharm Res 2018; 17(11):2189-2197 doi: 10.4314/tjpr.v17i11.12

© 2018 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the cardioprotective role of salvianolic acid B (SAB-B) against isoproterenol (ISO)-induced cardiotoxicity in a rat model.

Methods: A total of 32 male rats were equally divided into four groups as follows: control group given saline only; rats treated with ISO (85 mg/kg; i.p.) for 2 consecutive days (MI model; ISO group); rats pre-treated with SAB-B (20 mg/kg, i.p.) prior to exposure to ISO (SAB-B+ISO, treatment group), and rats administrated SAB-B (20 mg/kg, i.p.) for 28 days (SAB-B group).

Results: Pre-treatment with SAB-B for 28 days enhanced the levels of various antioxidants (CAT, SOD and GSH) and hemodynamic parameters, viz, systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial pressure (MAP). Likewise, substantial decrease in the levels of lipid peroxidation product (MDA), inflammatory markers (NF-κb, TNF-α, IL-1β and IL-6), heart weight, heart-to-body weight ratio, and serum cardiac markers (CPK and LDH) were seen in SAB-B treated rats, relative to ISO-treated group. Rats in SAB-B group had significantly downregulated protein expressions of NF-κb and TNF-α, and significant reduction in histopathological changes (edema and necrosis) after 28 days of treatment.

Conclusion: SAB-B possesses potent cardio-protective potential against ISO-induced cardiotoxicity due to its antioxidant, anti-inflammatory and anti-necrotic properties.

Keywords: Salvianolic acid B, Isoproterenol, Antioxidants, Inflammatory markers, Cardioprotection