Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Optimization of L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia

Maria Morales-Gonzalez, Boghos Stepanian Martinez, Laura Ramirez-Rodriguez, J EC Gómez, L E Diaz

La Sabana University, Engineering Faculty, Campus del Puente del Común, Km 7 Autopista Norte de Bogotá. Chia, Colombia;

For correspondence:- L Diaz Email: luisdb@unisabana.edu.co

Accepted: 19 October 2018 Published: 30 November 2018

Citation: Morales-Gonzalez M, Martinez BS, Ramirez-Rodriguez L, Gómez JE, Diaz LE. Optimization of L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia. Trop J Pharm Res 2018; 17(11):2199-2206 doi: 10.4314/tjpr.v17i11.13

© 2018 The authors.
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Abstract

Purpose: To optimize the L-asparaginase activity of Actinobacteria isolated from Guaviare river sediments in Colombia.

Methods: Actinobacterial strains were evaluated for their L-asparaginase activity using phenol red plates and Nessler’s assays. Strains with L-asparaginase activity were identified based on 16S ribosomal rRNA sequencing, and a central composite design was used to study nutritional and growth factors that could improve L-asparaginase activity. L-asparaginase protein was detected using western blotting and the cytotoxicity of L-asparaginase preparations was evaluated against MDA-MB231 and L929 cell lines.

Results: Kitasatospora atroaurantiaca, Streptomyces griseoluteus, and Streptomyces panaciradicis were cultured in medium with lactose as a carbon source and a combination of asparagine and malt extract as nitrogen sources. These strains showed L-asparaginase activities of 29.4, 114.06, and 34.08 U/mg, respectively, and half-maximal inhibitory concentration (IC₅₀) values of 25.61 ± 2.15, 8.18 ± 1.61, and 165.29 ± 1.06 ppm, respectively, against MDA-MB 231 cells. Western blotting analysis revealed the presence of an L-asparaginase monomer with a molecular weight of 37 kDa.

Conclusion: Kitasatospora atroaurantiaca, Streptomyces griseoluteus, and Streptomyces Panaciradicis produce L-asparaginases with low L-glutaminase activity and promising cytotoxic activity and thus may be useful for the management of acute lymphoblastic leukemia.

Keywords: L-asparaginase, Streptomyces, Kitasatospora, Optimization, Cytotoxicity