Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Identification of multi-drug resistant genes in P. aeruginosa isolates from patients under mechanical ventilation and respiratory support in an intensive care unit

Xiaoli Yang¹, Hongping Yao², Chun Zhang³, Cui Li¹, Congying He¹, Jinfang Sheng¹

¹Shaanxi Institute for Food and Drug Control, Xiâ€™an, 710065; ²Department of Pharmacy; ³Department of Surgical Intensive Care Unit, The First Affiliated Hospital of Xiâ€™an Jiaotong University, Xiâ€™an 710061, China.

For correspondence:- Jinfang Sheng Email: infangsheng@hotmail.com Tel:+862962288433

Accepted: 19 October 2018 Published: 30 November 2018

Citation: Yang X, Yao H, Zhang C, Li C, He C, Sheng J. Identification of multi-drug resistant genes in P. aeruginosa isolates from patients under mechanical ventilation and respiratory support in an intensive care unit. Trop J Pharm Res 2018; 17(11):2227-2234 doi: 10.4314/tjpr.v17i11.17

© 2018 The authors.
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Abstract

Purpose: To determine multi-drug resistant (MDR) and metallo β-lactamase (MBL)-resistant genes from Pseudomonas aeruginosa isolated from intensive care unit (ICU) patients under mechanical ventilation and respiratory support.

Methods: P. aeruginosa was isolated from 387 purulent tracheobronchial secretions collected from ICU patients who were intubated and mechanically ventilated for at least 48 h. Antibiotic resistance was determined by minimum inhibitory concentration (MIC) assay while MDR genes, viz, blaTEM, blaOXA, blaVIM, blaCTX-M-15 were determined by polymerase chain reaction (PCR).

Results: A total of 144 (37.2 %) P. aeruginosa were isolated from the purulent tracheobronchial secretions. A majority of the isolates (51.4 %) were resistant to gentamicin. Meropenem-gentamicin was the predominant (35.4 %) resistant combination. Out of the 144 isolates, 102 (70.8 %) were positive for blaTEM gene, 51 (35.4 %) for were positive for blaOXA gene, 22 (15.3 %) were positive for blaVIM gene, while 19 (13.2 %) were positive for blaCTX-M gene.

Conclusion: The high prevalence of MDR P. aeruginosa indicates the need for continued monitoring of MDR P. aeruginosa especially in ICU patients who are under mechanical respiratory support.

Keywords: Multi-drug resistance genes, Mechanical ventilator, Respiratory support, Pseudomonas aeruginosa