Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Allelic frequency of PON1 Q192R, CYP2C192 and CYP2C1917 among Jordanian patients taking clopidogrel

Malek Rjoub¹, Akram Saleh², Nancy Hakooz³, Amer Imraish⁴, Yazun Jarrar⁵, Malek Zihlif¹

¹Department of Pharmacology, Faculty of Medicine; ²Department of Internal Medicine, University of Jordan Hospital; ³Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy; ⁴Department of Biology, Faculty of Science, The University of Jordan; ⁵Department of Pharmacy, College of Pharmacy, AlZaytoonah University of Jordan, Amman, Jordan.

For correspondence:- Malek Zihlif Email: M.zihlif@ju.edu.jo Tel:+96265355000

Accepted: 23 October 2018 Published: 30 November 2018

Citation: Rjoub M, Saleh A, Hakooz N, Imraish A, Jarrar Y, Zihlif M. Allelic frequency of PON1 Q192R, CYP2C19*2 and CYP2C19*17 among Jordanian patients taking clopidogrel. Trop J Pharm Res 2018; 17(11):2275-2280 doi: 10.4314/tjpr.v17i11.24

© 2018 The authors.
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Abstract

Purpose: To investigate the influence of allelic frequencies of PON1 Q192R, CYP2C19*2 and CYP2C19*17 genetic polymorphisms on the response to clopidogrel among Jordanian patients.

Methods: Polymorphisms in CYP2C19 were assessed among 148 patients using PCR-RFLP assay.

Results: The CYP2C19*2, CYP2C19*17, and PON1 Q192R allele frequencies were 9.8, 28.72 and 28.7 %, respectively. On the genotyping side, the frequencies of CYP2C19*1/1* and CYP2C19*1/2* were 80.4 and 19.6 %, respectively, but none of the patients had CYP2C19*2/2* genotype. The genotype frequencies CYP2C19*17 were 47.97, 46.62 and 5.41 % for wild-type C-C, heterozygote C-T, and the mutant T-T, respectively. PON1 genotype was 42.7 % for QQ, and 57.8 % for QR. None of the patients had RR genotype.

Conclusion: Relative to other populations, the observed allelic frequencies are consistent with the values reported for Caucasian and Middle Eastern populations.

Keywords: CYP2C9 polymorphisms, Clopidogrel, Genotype, Allele frequency, PON1 genes