Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation

Alexander Patera Nugraha¹, Ida Bagus Narmada², Diah Savitri Ernawati⁴, Aristika Dinaryanti³, Eryk Hendrianto³, Igo Syaiful Ihsan³, Wibi Riawan⁵, Fedik Abdul Rantam^3,5

¹Doctoral Student of Medical Science, Faculty of Medicine, Universitas Airlangga; ²Orthodontic Department; ³Stem Cell Research and Development Center, Universitas Airlangga, Surabaya; ³Oral Medicine Department, Faculty of Dental Medicine; ⁴Biochemistry Biomolecular Laboratory, Faculty of Medicine, Universitas Brawijaya; ⁵Virology and Immunology Laboratory, Microbiology Department, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia;

For correspondence:- Fedik Rantam Email: fedik-a-r@fkh.unair.ac.id

Accepted: 20 November 2018 Published: 26 December 2018

Citation: Nugraha AP, Narmada IB, Ernawati DS, Dinaryanti A, Hendrianto E, Ihsan IS, et al. In vitro bone sialoprotein-I expression in combined gingival stromal cells and platelet rich fibrin during osteogenic differentiation. Trop J Pharm Res 2018; 17(12):2341-2345 doi: 10.4314/tjpr.v17i12.4

© 2018 The authors.
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Abstract

Purpose: To analyze the expression of bone sialoprotein - I (BSP - I) after the addition of platelet rich fibrin (PRF) in gingival somatic cell (GSC) culture medium during osteogenic differentiation in vitro.

Methods: GSCs were extracted from healthy, 1-month-old, male Wistar rats (Rattus Novergicus), weighing 250 - 300 g, and which had been randomly selected (n=4). These cells were cultured for 14 days and passaged every 4 days. Five subcultures of GSCs were cultured in three plates (M24) (N = 54; n = 6) for 7, 14 and 21 days in three preconditioned culture media (group I: plain culture media; group II: preconditioned osteogenic culture media, and group III: preconditioned osteogenic culture media with platelet rich fibrin). The expression of BSP-I was immunocytochemically (ICC) examined with monoclonal antibodies. Homogeneity and normality tests (p > 0.05) were then performed followed by an analysis of variance (ANOVA, p < 0.05).

Results: The highest expression of BSP-I was found in group III (Day 21, 13.00 ± 2.000), while the lowest expression of BSP-I was found in group I (Day 7, 7.33 ± 1.155). There were significant differences between the groups (p = 0.000, p < 0.05).

Conclusion: PRF stimulates and significantly enhances the expression of BSP-I in GSC culture during osteogenic differentiation. Thus, PRF can be used to accelerate regeneration of alveolar bone defects.

Keywords: Alveolar bone, Bone Sialoprotein-I, Gingival Somatic cells, Osteogenic ability, Platelet rich fibrin