Meltem Uçar1 
,
Orhan Deger2
1Faculty of Health Science, European University of Lefke, Lefke, Northern Cyprus, TR-10 Mersin;
2Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey.
For correspondence:- Meltem Uçar
Email: mucar@eul.edu.tr Tel:+903936602000
Accepted: 20 January 2019
Published: 28 February 2019
Citation:
Uçar M, Deger O.
Evaluation of cytotoxic and wound healing effect of DMEM extracts of Turkish propolis in MDA-MB-231 cell lines. Trop J Pharm Res 2019; 18(2):321-325
doi:
10.4314/tjpr.v18i2.14
© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Abstract
Purpose: To investigate the effect of Dulbecco's Modified Eagle Medium (DMEM) extract of Turkish propolis on proliferation, cytotoxicity and lateral motility in MDA-MB-231 cells.
Methods: The antiproliferative activity of DMEM extracts of propolis was determined colorimetrically in MDA-MB-231 cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Cell toxicity and wound healing effects of the prpolis extracts were determined with trypan blue exclusion assay and wound-healing assay, respectively.
Results: The cell number of MDA-MB-231 cells were decreased by the extracts at all concentrations for 72 h. The highest antiproliferative activity of the extract was demonstrated at 10 mg/mL for 24 - 72 h. Moreover, 5 and 0.31 mg/mL of the propolis extract showed significant antiproliferative activity at 72 h of incubation. The extract showed cytotoxic effect to MDA-MB-231 cells at 10 mg/mL. The extract (at a dose of 2.5 mg/mL) during 24 - 72 h did not produce any effect on lateral motility in MDA-MB-231 cells in the wound healing assay.
Conclusion: These results indicate that the DMEM extract of propolis exerts antiproliferative and cytotoxic effects on MDA-MB-231 cells at different concentrations.
Keywords: Propolis, Proliferation, Cytotoxicity, Motility, Breast cancer
Fulltext in PDF
