Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

MicroRNA-124 regulates apoptosis in sevoflurane anesthesia-induced neuroblastoma cells by targeting enhancer of zeste homolog 2

Lingyun Wei, Jiansheng Fang, Yiqi Fang, Chengnv Li

Department of Anesthesiology, Chunan First People's Hospital, Chunan, Zhejiang, 311700, China;

For correspondence:- Chengnv Li Email: lichengnvll@163.com Tel:+8657165025815

Accepted: 25 February 2019 Published: 31 March 2019

Citation: Wei L, Fang J, Fang Y, Li C. MicroRNA-124 regulates apoptosis in sevoflurane anesthesia-induced neuroblastoma cells by targeting enhancer of zeste homolog 2. Trop J Pharm Res 2019; 18(3):491-497 doi: 10.4314/tjpr.v18i3.7

© 2019 The authors.
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Abstract

Purpose: To investigate the mechanism of microRNA-124 action on neuroblastoma apoptosis induced by sevoflurane.

Methods: MiR-124 expression was assessed in a neuroblastoma cell line (SMS-KAN) using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The role of miR-124 in sevoflurane anesthesia-induced neuroblastoma was studied by cell activity and apoptosis analysis using 3-(4, 5-dimethylthiazolyl-2-yl)-2-5 diphenyl tetrazolium bromide (MTT) assay and flow cytometry, respectively. MiR-124 target protein genes were confirmed via luciferase reporter activity, qRT-PCR, and western blot analysis.

Results: miR-124 was upregulated in sevoflurane anesthesia-induced neuroblastoma (p < 0.05). After miR-124 knockdown, apoptosis was significantly reduced and cell viability was enhanced in sevoflurane anesthesia-induced SMS-KAN nerve cells (p < 0.05). Furthermore, a significant reduction of luciferase activity was observed in 293T cells co-transfected with miR-124 mimics and EZH2-wild type (EZH2-WT) (p < 0.05). The mRNA and protein expression levels of EZH2 decreased in SMS-KAN nerve cells transfected with miR-124 mimics (p < 0.05). Overexpression of EZH2 inhibited the apoptosis of SMS-KAN cells induced by sevoflurane (p < 0.05). Furthermore, the apoptosis of SMS-KAN cells transfected with miR-124 inhibitor were offset by transfected siEZH2.

Conclusion: The results suggest that overexpression of miR-124 suppresses cell proliferation by targeting EZH2 in SMS-KAN cells. Therefore, miR-124 represents a potential target for neuroblastoma therapy.

Keywords: Sevoflurane, Anesthesia, Neuroblastoma, Neurotoxicity, MiR-124, enhancer of zeste homolog 2 (EZH2), Apoptosis