Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antidiabetic effect of hydro-methanol extract of Prunus cerasus L fruits and identification of its bioactive compounds

Gong Xiao, Xiangcheng Xiao

Department of Nephrology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China;

For correspondence:- Xiangcheng Xiao Email: BicklerElese@yahoo.com Tel:+8613787312910

Accepted: 19 February 2019 Published: 31 March 2019

Citation: Xiao G, Xiao X. Antidiabetic effect of hydro-methanol extract of Prunus cerasus L fruits and identification of its bioactive compounds. Trop J Pharm Res 2019; 18(3):597-602 doi: 10.4314/tjpr.v18i3.22

© 2019 The authors.
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Abstract

Purpose: To investigate the antidiabetic effect of hydro-methanol extract of Prunus cerasus fruit extract.

Methods: The antidiabetic activity was assessed in alloxan-induced diabetic rats. The effect of P. cerasus fruit extract on plasma fasting blood glucose (FBG), insulin, C-peptide, total protein, glycosylated hemoglobin (HbA1c), total hemoglobin, reduced glutathione (GSH), vitamins E and C, ceruloplasmin, lipid profile, histology of the pancreas, and expression of glucose transporter type 4 (GLUT-4) were determined using standard procedures. Liquid chromatography-mass spectrometry was used for phytochemical analysis.

Results: Alloxan-induced diabetes significantly reduced plasma levels of insulin, C-peptide, total hemoglobin and total protein, and significantly increased FBG and HbA1c levels (p < 0.05). However, after treatment with the extract, changes in the levels of these parameters were significantly and dose-dependently reversed (p < 0.05). The extract also increased the levels of GSH, vitamins E and C, and. Alloxan-induced DM significantly increased the levels of triacylglycerols (TGs), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C), and significantly reduced the level of total cholesterol (TC) at different time points (p < 0.05). However, after treatment, the levels of TG, LDL-C and HDL-C declined but TC level was significantly elevated time- and dose-dependently by the extract (p< 0.05). The extract upregulated the expression of GLUT-4 mRNA in soleus muscle and adipose tissue. LC-MS analysis revealed that the extract contained chlorogenic acid, rutin, diadzin, amygdalin, quercetin and naringenin.

Conclusion: The results obtained in this study have shown that hydromethanol extract of P. cerasus fruits exhibits remarkable antidiabetic effects.

Keywords: Prunus cerasus, Diabetes mellitus, Alloxan, Fasting blood glucose, Oxidative stress