Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Bioactivity, nutritional property, and rapid chemical characterization of aqueous extract of Annona muricata leaf from Mexico

Ana V Coria-Téllez¹ , Eva N Obledo-Vázquez¹, Eduardo Padilla-Camberos², Marisela González-Ávila², Moisés Martínez-Velázquez²

¹Laboratorio de Análisis y Diagnóstico del Patrimonio, El Colegio de Michoacán, A.C., 59379, Michoacán; ²Centro de Investigación y Asistencia en Tecnologia y Diseño del Estado de Jalisco, A.C., 44270, Jalisco, México.

For correspondence:- Ana Coria-Téllez Email: anac@colmich.edu.mx Tel:+523525256107

Accepted: 19 February 2019 Published: 31 March 2019

Citation: Coria-Téllez AV, Obledo-Vázquez EN, Padilla-Camberos E, González-Ávila M, Martínez-Velázquez M. Bioactivity, nutritional property, and rapid chemical characterization of aqueous extract of Annona muricata leaf from Mexico. Trop J Pharm Res 2019; 18(3):611-617 doi: 10.4314/tjpr.v18i3.24

© 2019 The authors.
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Abstract

Purpose: To investigate the bioactive and nutritional properties, as well as rapid chemical characterization of aqueous extract of Annona muricata leaf from Mexico

Methods: The crude aqueous extract of A. muricata leaf was obtained by decoction. Cytotoxicity was tested against cervicouterine cancer cells (HeLa) using methyl thiazol tetrazolium (MTT) assay. Antioxidant activity was evaluated using 2, 2-diphenylpicrylhydrazyl (DPPH) assay. Nutritional evaluation was carried out according to Association of Official Analytical Chemists (AOAC) procedures. Rapid qualitative chemical characterization of the extract was carried out by direct analysis in real-time mass spectrometry (DART-MS) method.

Results: The aqueous extract of A. muricata leaf showed cytotoxicity against HeLa cells and also antioxidant activity in a concentration-dependent manner. Nutritional analysis revealed the presence of carbohydrates, vitamin C, Na, and Fe in the aqueous extract. DART-MS spectra showed the presence of alkaloids and phenols as the major components.

Conclusion: The cytotoxic and antioxidant activities of the aqueous extract of A. muricata leaf lend some support for its traditional uses as anti-cancer remedy. These activities are probably due to its active secondary metabolites. Thus, the aqueous extract is a source of healthy nutritional components as well as a potential anti-cancer agent for humans.

Keywords: Annona muricata, Antioxidant activity, Cytotoxicity, DART-MS, Nutritional composition, Sour sop