Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Involvement of HAND1 and CBS in maintenance of cardiac micro-architecture following obesity-induced heart failure

Xiandong Sun¹, Jian Cao², Haiming Tong³, Xizhe Zhang⁴, Yi Sun⁴, Zhimin Wei¹, Ronghai Man¹

¹Department of Cardiology; ²Department of Orthopedics; ³Department of Emergency; ⁴Department of Anesthesiology, Chifeng Municipal Hospital, Chifeng, Inner Mongolia, 024000, China.

For correspondence:- Ronghai Man Email: RClelandechen@yahoo.com Tel:+864768352060

Accepted: 23 March 2019 Published: 30 April 2019

Citation: Sun X, Cao J, Tong H, Zhang X, Sun Y, Wei Z, et al. Involvement of HAND1 and CBS in maintenance of cardiac micro-architecture following obesity-induced heart failure. Trop J Pharm Res 2019; 18(4):747-752 doi: 10.4314/tjpr.v18i4.10

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To study the role of heart and neural crest derivatives expressed 1 (HAND1) and cystathionine-beta-synthase (CBS) in the maintenance of cardiac architecture following high fat diet-induced obesity.

Methods: Mouse models of initial and critical heart disease were established by continuous feeding of high fat diet for 7 and 12 months, respectively. The expression of HAND1 and CBS were assayed using immunohistochemistry and Western blotting.

Results: Obesity led to mild and severe forms of heart disease which were confirmed through histological imaging. Initial obesity resulted in cardiac tissue remodeling along with initial degeneration, while critical obesity resulted in tissue hardening. The expression of HAND1 was upregulated 4.3 folds in the mild form of cardiac failure, relative to marginal expression pattern of HAND1 in control tissue. However, as the disease progressed, the expression of HAND1 was limited in serve form of cardiac failure. Moreover, the expression of cystathionine beta-synthase (CBS) was upregulated 3.7-fold in the initial form of heart failure, but was subsequently reduced in serve form of heart disease.

Conclusion: These results reveal that in high fat diet-induced cardiac stress, the over-expressions of HAND1 and CBS at the initial stages induce extensive alterations in cardiac architecture.

Keywords: Obesity, High-fat diet, Heart and Neural Crest Derivatives Expressed 1, Cystathionine-beta-synthase, Cardiac re-modeling