Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Boeravinone B promotes fracture healing in ovariectomy-induced osteoporotic rats via the regulation of NF-κB p65/IκB-α/SIRT-1 signaling pathway

Jianlin Zhang, Longze Zong , Dongyu Bai

Department of Joint Surgery, Yan'an University Affiliated Hospital, Yan'an 716000, China;

For correspondence:- Longze Zong Email: AnnieaJohnsonol@yahoo.com Tel:+861518979510

Accepted: 26 April 2019 Published: 31 May 2019

Citation: Zhang J, Zong L, Bai D. Boeravinone B promotes fracture healing in ovariectomy-induced osteoporotic rats via the regulation of NF-κB p65/IκB-α/SIRT-1 signaling pathway. Trop J Pharm Res 2019; 18(5):955-960 doi: 10.4314/tjpr.v18i5.7

© 2019 The authors.
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Abstract

Purpose: To investigate the fracture-healing effect of boeravinone B in ovariectomy-induced (OVX) osteoporotic rats.

Methods: Adult female Wistar rats (n = 30) were ovariectomized and after three months, the unilateral cross-tibial fractures were fixed with intramedullary nails. The rats were then randomly assigned to three groups of 10 rats each: normal control group, OVX group and 100 mg/kg body weight boeravinone B group. Boeravinone B was orally administered for a period of 5 weeks. The effect of boeravinone B on indices of bone formation and resorption was assessed. Levels of inflammatory cytokines including tumor necrosis factor- α (TNF-α) and interleukin-1β (IL-1β) were determined using enzyme-linked immunosorbent assay (ELISA). Western blotting was used to determine the expression levels of NF-κB p65, Il2;B-α and SIRT1 proteins.

Results: There were significant increases in the activities of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP), and collagen type I fragment (CTX) level and serum osteocalcin (OC) of OVX group, when compared with normal control group (p < 0.05). However, treatment with boeravinone B significantly reduced the activities and levels of these parameters, relative to OVX group (p < 0.05). The levels of TNF-α and IL-1β significantly increased in OVX group, relative normal control group, but were significantly lower following treatment with boeravinone B (p < 0.05). Bone mineral content (BMC) was not significantly altered in OVX and boeravinone B-treated groups, when compared with normal control group (p > 0.05). There was significant reduction in bone mineral density (BMD) of OVX group relative to normal control group (p < 0.05). However, treatment with boeravinone B significantly increased the BMD, when compared with OVX group (p < 0.05). After Week 5 of treatment, boeravinone B significantly enhanced bone remodeling and formation of callus. Treatment with boeravinone B significantly reduced the expression levels of NF-κB p65 and IκB-α proteins, and significantly upregulated the expression of SIRT-1 (p < 0.05).

Conclusion: The results obtained in this study suggest that boeravinone B promotes the healing of fracture caused by osteoporosis via a mechanism involving NF-κB p65/IκB-α/SIRT-1 signaling pathway.

Keywords: Osteoporosis, Boeravinone B, Bone fracture, Ovariectomy, Inflammatory cytokines