Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Total flavones of Desmodium styracifolium antagonize calcium oxalate monohydrate-triggered IL-2Rβ expression in renal epithelial cells

Haijie Xie, Rui Yang, Yong Xu, Chunyu Liu

Urolithiasis Treatment Center of Tianjin, Tianjin Institute of Urology, The Second Hospital of Tianjin Medical University, 23 Pingjiang Road, Hexi District, Tianjin 300211, China;

For correspondence:- Chunyu Liu Email: chunyuliu2017@126.com Tel:+862288328091

Accepted: 22 April 2019 Published: 31 May 2019

Citation: Xie H, Yang R, Xu Y, Liu C. Total flavones of Desmodium styracifolium antagonize calcium oxalate monohydrate-triggered IL-2Rβ expression in renal epithelial cells. Trop J Pharm Res 2019; 18(5):1077-1081 doi: 10.4314/tjpr.v18i5.23

© 2019 The authors.
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Abstract

Purpose: To explore the effect of total flavone of Desmodium styracifolium (TFDS) on calcium oxalate monohydrate (COM)-triggered IL-2Rβ expression in human kidney proximal tubular epithelial cells.

Methods: Human kidney proximal tubular epithelial cell line HK-2 was treated with COM, TFDS or both. The expression of IL-2Rβ was evaluated by quantitative polymerase chain reaction (qPCR) or flow cytometry. The responsiveness of HK-2 cells to IL-2 was determined by enzyme-linked immunosorbent assay (ELISA), qPCR and western blot. The signaling mechanism underlying the effect of TFDS was studied using western blot and qPCR. The clinical relevance of IL-2Rβ to renal inflammation was investigated by re-analyzing a Gene expression Omnibus (GEO) dataset.

Results: Total flavones of Desmodium styracifolium (TFDS) antagonize COM-triggered IL-2Rβ expression in HK-2 cells, thus reducing the responsiveness of HK-2 cells to IL-2 stimulation. Mechanistically, TFDS dampens IL-2Rβ expression by preventing the activation of STAT3. The level of IL-2Rβ is positively correlated with the inflammatory status of the kidney.

Conclusions: The total flavones of Desmodium styracifolium (TFDS) prevent the upregulation of IL-2Rβ in renal epithelial cells upon COM stimulation in a STAT3-dependent manner.

Keywords: Total flavones, Desmodium styracifolium, Calcium oxalate, Urinary calculi, IL-2R^6;, STAT3 signaling