Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Seven-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one reduces atherogenic index and Nrf2 and GPx gene expressions in hyperlipidemic rats

Prasetyastuti ¹ , Rahmah Dara Ayunda², Sunarti ¹

¹Faculty of Medicine, Public Health and Nursing; ²Biomedical Science, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.

For correspondence:- Prasetyastuti Email: prasetyastuti.djati@gmail.com Tel:+6281227882379

Accepted: 20 May 2019 Published: 30 June 2019

Citation: P, Ayunda RD, S. Seven-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one reduces atherogenic index and Nrf2 and GPx gene expressions in hyperlipidemic rats. Trop J Pharm Res 2019; 18(6):1173-1177 doi: 10.4314/tjpr.v18i6.4

© 2019 The authors.
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Abstract

Purpose: To investigate the effect of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one isolated from mahogany (Swietenia macrophylla King) seeds on atherogenic index, expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) and expression of the glutathione peroxidase (GPx) genes in hyperlipidemic rats.

Methods: A total of 25 rats male aged 8 weeks and weighing an average of 200 g were used. They were divided into five groups as follows: (I) normal (N), (II) hyperlipidemic (HL), (III) hyperlipidemic rats treated with simvastatin (HL+SV), (IV and V) hyperlipidemic rats treated with 30 or 90mg, respectively, of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one per 200 g body weight per day for 4 weeks. Atherogenic index (AI) was calculated from the levels of triglyceride (TG) and high-density lipoprotein (HDL) while Nrf2 and GPx gene expressions were determined by quantitative real-time polymerase chain reaction (qRT-PCR).

Results: Two different doses of 7-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one in hyperlipidemic rats significantly reduced their atherogenic index (p < 0.05). Nrf2 and GPx expression levels were lower than (p > 0.05) those of hyperlipidemic group.

Conclusion: Seven-hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one reduces the atherogenic index and expression levels of Nrf2 and GPx genes in hyperlipidemic rats. Thus, this compound has potential as an antihyperlipidemic agent.

Keywords: Hyperlipidemia, Oxidative stress, Nrf2, GPx, Gene expression