Lin Wang1,
Runyong Fan1,
Funeng Geng2,
Yongxiang Gao3,
Qinwan Huang1 
For correspondence:- Qinwan Huang Email: hqwan2163@163.com
Accepted: 28 May 2019 Published: 30 June 2019
Citation: Wang L, Fan R, Geng F, Gao Y, Huang Q. Protective effect of crude polysaccharide from Pao-Tian-Xiong derived from monkshood, against chronic renal failure in mice. Trop J Pharm Res 2019; 18(6):1235-1243 doi: 10.4314/tjpr.v18i6.13
© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the effect of crude polysaccharide isolated from pao-tian-xiong on chronic renal failure in mice, and its monosaccharide composition.
Methods: Male Kunming mice were orally treated with adenine (211.5 mg/kg/day) for 7 days, followed by either crude polysaccharides (125, 250 or 500 mg/kg), or positive drug solution (jinguishenqi pill, 2000 mg/kg) for another 7 days (each group had 15 mice). Mice in normal and negative control groups were given saline. Mental and physical states, blood urine nitrogen (BUN) and serum creatinine (SCr), kidney morphological changes and organ indices were determined. Histopathological examination of spleen and kidney tissues was also performed. The monosaccharide composition of crude polysaccharide was determined by high-performance liquid chromatography (HPLC) and gas chromatography and mass spectrometry (GC-MS).
Results: Compared with negative control group, serum BUN (6.71 mmol/L vs. 8.61 mmol/L) and Cr (107.74 vs. 113.39 μmol/L) were significantly decreased by the crude polysaccharide isolate (p < 0.05), whereas epididymis index (0.2556 vs. 0.2135 %) and seminal vesicle index (0.5547 vs. 0.3945 %) were increased (p < 0.05). Histopathological examination showed that injuries to kidney, spleen, testis and epididymis decreased significantly. The crude polysaccharides contained mainly glucose, rhamnose, arabinose, galactose, mannose, galacturonic acid, glucuronic acid and xylose, and their contents ranged from 0.7 to 65 %.
Conclusion: These results suggest that the crude polysaccharides of Pao-tian-xiong ameliorates CRF symptoms in mice, thereby providing experimental evidence in support of its use as an anti-CRF drug.
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