Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Investigation of in vivo protective effect of orally administered vitamin E and selenium against gentamicin-induced renal and hepatic toxicity

Amin Alper Al-Doaiss^1,2, Yazun B Jarrar³

¹Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia; ²Anatomy and Histology Department, Faculty of Medicine, Sana'a University, Sana'a, Republic of Yemen; ³Department of Pharmacy, College of Pharmacy, Alzaytoonah University of Jordan, Amman, Jordan.

For correspondence:- Yazun Jarrar Email: yazun.jarrar@zuj.edu.jo Tel:+962795930283

Accepted: 21 June 2019 Published: 28 July 2019

Citation: Al-Doaiss AA, Jarrar YB. Investigation of in vivo protective effect of orally administered vitamin E and selenium against gentamicin-induced renal and hepatic toxicity. Trop J Pharm Res 2019; 18(7):1435-1442 doi: 10.4314/tjpr.v18i7.10

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the protective effect of vitamin E (Vit E) and selenium (Se) combination against gentamycin (GM)-induced renal and hepatic toxicity in rats.

Methods: Forty-eight male Wistar albino rats were administrated GM at a dose of 80 mg/kg/day, with or without Se (1.5 mg/kg/day), and/or Vit E (250 mg/kg/day) for a period of 4 weeks. Serum samples from each rat were subjected to biochemical analysis for kidney and liver functions, while kidney and liver biopsies were also investigated by histological examination.

Results: GM significantly increased serum creatinine, urea, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and free radicals (p < 0.05). Moreover, GM induced significant histological and ultrastructural alterations in the renal and hepatic tissues of the rats. Exposure to a combination of Vit E and Se did not attenuate the GM-induced toxicity in renal and hepatic tissues.

Conclusion: These results suggest that Vit E and Se combination have no significant protective role against GM-induced hepatic and renal toxicity.

Keywords: Antioxidants, Vitamin E, Selenium, Gentamicin, Toxicity