Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots

Nacer Amraoui, Nozha Mayouf, Noureddine Charef, Abderrahmane Baghiani, Lekhmici Arrar

Laboratory of Applied Biochemistry, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif 1, Setif 19000 Algeria;

For correspondence:- Lekhmici Arrar Email: lekharrar@hotmail.com Tel:+213666466220

Accepted: 24 June 2019 Published: 29 July 2019

Citation: Amraoui N, Mayouf N, Charef N, Baghiani A, Arrar L. Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots. Trop J Pharm Res 2019; 18(7):1499-1506 doi: 10.4314/tjpr.v18i7.20

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the antioxidant, anti-inflammatory and antiR08;arthritic effects of the methanol extract of Tamus communis roots.

Methods: The total phenolic and flavonoid contents were determined using Folin–Ciocalteu’s reagent and aluminium chloride assays, respectively while the antioxidant capacity was determined using DPPH and β-carotene/linoleate tests. For anti-inflammatory activity, carrageenan-induced paw edema in rat was used. Formaldehyde-induced paw edema and adjuvant-induced arthritis in rats were used to evaluate the anti-arthritic effect of the extract.

Results: The total phenolic and flavonoid contents of T. communis showed 55.2 ± 0.036 mg GAE/g dry weight and 1.191 ± 0.002 mg QEq/g dry weight, respectively while the antioxidant activity (DPPH) showed a half-maximal inhibitory concentration (IC₅₀ of 0.128 ± 0.011 mg/mL. Using β-carotene/linoleate assay, the extract showed 88.13 ± 4.03 % inhibition. In carrageenan-induced paw edema in rat, all doses of T. communis did not show inhibition of edema. In contrast, formaldehyde-induced paw edema decreased at the dose of 150 mg/kg days 2 and 4. However, doses of 300 and 600 mg/kg did not show any activity. In adjuvant-induced arthritis, all used doses caused no reduction in arthritic scores and in paw thickness except a significant decrease with the dose 150 mg/kg at the last day.

Conclusion: T. communis extract exhibits high antioxidant activity related to the phenolic compound contents. However, the results of the anti-inflammatory and antiR08;arthritic studies did not support its use in folk medicine in the treatment of rheumatic ailment except on low doses

Keywords: Tamus communis, DPPH, Formaldehyde-induced paw edema, Adjuvant-induced arthritis, Anti-inflammatory, AntiR08;arthritic