Nael Abutaha 
,
Ashraf MA Mashaly,
Muhammad Farooq,
Muhammad A Wadaan
For correspondence:- Nael Abutaha Email: naelabutaha@yahoo.com Tel:+96591912845
Received: 24 March 2015 Accepted: 29 August 2015 Published: 31 October 2015
Citation: Abutaha N, Mashaly AM, Farooq M, Wadaan MA. Apoptotic potential of Artemsia sieberia Besser (Asteraceae) fraction against human cancer cell lines. Trop J Pharm Res 2015; 14(10):1779-1785 doi: 10.4314/tjpr.v14i10.7
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the anti-proliferative and apoptotic activity of crude and dichloromethane fraction of A. sieberi against seven cancer cell lines (Colo20, HCT116, DLD, MCF7, Jurkat, HepG2 and L929).
Methods: A. sieberi was extracted with methanol and further purification was carried out using liquid-liquid extraction with hexane, dichloromethane and ethyl acetate. Each extract was assayed for cytotoxic potential against cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (MTT) assay. The morphology of the HepG2 cell nucleus was investigated by Hoechst 33342, DNA-binding dye. A Tali™ image-based cytometer was used to assess cell viability, death and apoptosis using annexin-v /pi (propidium iodide). A chromatographic fingerprint was constructed using high performance liquid chromatography (HPLC).
Results: The most effective anticancer activity of the unrefined methanol extract was against HepG2 cell lines (LC50 = 161.5 µg/mL). The hexane and ethyl acetate fractions showed no antiproliferative activity. The dichloromethane fraction displayed higher cytotoxic activity (LC50 = 61.75 µg/mL) and also repressed the migration of the cells. About 50 % of HepG2 cells were apoptotic when treated for 24 h with the dichloromethane fraction at the concentration of 120 µg/mL
Conclusion: A. sieberi possesses apoptotic activity and inhibited the migration of the HepG2 cell lines.
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