Fatma Pehlivan Karakas1,
Arzu Birinci Yildirim1,
Recep Bayram3,
Muhsine Z Yavuz3,
Akcahan Gepdiremen3,
Arzu Ucar Turker2 
For correspondence:- Arzu Turker Email: turker_a@ibu.edu.tr Tel:+903742541238
Received: 14 May 2015 Accepted: 5 September 2015 Published: 31 October 2015
Citation: Karakas FP, Yildirim AB, Bayram R, Yavuz MZ, Gepdiremen A, Turker AU. Antiproliferative activity of some medicinal plants on human breast and hepatocellular carcinoma cell lines and their phenolic contents. Trop J Pharm Res 2015; 14(10):1787-1795 doi: 10.4314/tjpr.v14i10.8
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To determine the phenolic composition and antiproliferative activity of 16 different extracts (hexane, dichloromethane, methanol and water) obtained from Bellis perennis, Convolvulus galaticus, Trifolium pannonicum and Lysimachia vulgaris on human breast cancer (MCF-7) and human hepatocellular carcinoma (HepG2/C3A) cell lines.
Methods: The aerial parts of the plants were successively extracted with hexane, dichloromethane, methanol and water using a Soxhlet apparatus. The phenolic content of the plants were determined by plants by high performance liquid chromatography (HPLC) while their antiproliferative activity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, a yellow tetrazole (MTT) assay.
Results: Among the tested extracts, the methanol extract of B. perennis showed the best anti-proliferative activity against MCF-7 cell line with IC50 (inhibiting 50 % of cell growth) value of 71.6 µg/mL. Furthermore, the dichloromethane extract of C. galaticus showed the best anti-proliferative activity against HepG2/C3A cell line with IC50 of 57.3 µg/mL. The HPLC data for the plant extracts showed the presence of the following phenolic compounds: gallic acid monohydrate, caffeic acid, rutin hydrate, luteolin-7-O-β-D glucoside, kaempferol, myricetin, quercetin, coumarin and apigenin.
Conclusion: The findings of this study indicate that there is some justification for the use of B. perennis and C. galaticus as traditional anticancer medicinal herbs.
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