Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

GC-MS metabolic profiling and anti-urease activity of non-polar fractions of Calligonum Polygonoides L. (Polygonaceae) and Crateva Adansonii DC. Prodr. (Capparaceae)

Irfan Pervaiz , Saeed Ahmad, Adeel Arshad, Umair Khurshid, Abdul Basit

Faculty of Pharmacy & Alternative Medicine, Islamia University, Bahawalpur, Pakistan;

For correspondence:- Irfan Pervaiz Email: irfanpharmacist@gmail.com Tel:+923349808970

Accepted: 22 August 2019 Published: 30 September 2019

Citation: Pervaiz I, Ahmad S, Arshad A, Khurshid U, Basit A. GC-MS metabolic profiling and anti-urease activity of non-polar fractions of Calligonum Polygonoides L. (Polygonaceae) and Crateva Adansonii DC. Prodr. (Capparaceae). Trop J Pharm Res 2019; 18(9):1955-1960 doi: 10.4314/tjpr.v18i9.25

© 2019 The authors.
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Abstract

Purpose: To determine the urease-inhibitory activity and chemical constituents of fractions of

Calligonum polygonoides and Crateva adansonii separated by physical properties.

Methods: The anti-urease activities of different fractions of the plants (methanol, n-hexane, CHCl₃, n-butanol) were evaluated using a standard procedure. The chemical constituents of the extracts with the highest urease-inhibitory activity were determined by gas chromatography-mass spectrometry.

Results: The n-hexane fractions of both plants had higher urease-inhibitory activity and a lower half-maximal inhibitory concentration (IC₅₀) than the other extracts. GC-MS evaluation revealed that n-hexane fraction of C. polygonoides was rich in fatty acids (39.36 %), sterols (22.29 %), long chain alkanes (98.5 %), and a few volatiles (5.26 %), while the n-hexane fraction of C. adansonii had high levels of alkanes (35.03 %), sterols (10.46 %), fatty acid esters (46.82 %), and triterpenes (23.76 %).

Conclusion: The n-hexane fractions of the plants demonstrate high urease-inhibitory activity. Thus, these plant-based anti-urease fractions can potentially serve as a starting point for the development of novel antibacterial agents with enhanced efficacy and reduced antibiotic resistance in the treatment of pathological conditions and infections associated with urease.

Keywords: Calligonum polygonoides, Crateva adansonii, Urease, Natural products, Antibiotics, Helicobacter pylori