Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of early exogenous supplementation of rhIGF-1 on oxygen-induced retinopathy in a mice model of prematurity, and on expressions of IGF-1 and VEGF

Xiangjun Wu, Hui Ye, Qiao Cai, Yuanxiang Ke, Danying Wang

Department of Pediatrics, Taizhou First People's Hospital, Taizhou 318020, People's Republic of China;

For correspondence:- Danying Wang Email: zjchs7@163.com

Accepted: 29 September 2019 Published: 30 October 2019

Citation: Wu X, Ye H, Cai Q, Ke Y, Wang D. Effect of early exogenous supplementation of rhIGF-1 on oxygen-induced retinopathy in a mice model of prematurity, and on expressions of IGF-1 and VEGF. Trop J Pharm Res 2019; 18(10):2019-2024 doi: 10.4314/tjpr.v18i10.3

© 2019 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of early exogenous supplementation of recombinant human insulin-like growth factor (rhIGF-1) on oxygen-induced mouse model of retinopathy of prematurity (ROP).

Methods: Three groups of healthy SPF grade C57BL/6 mice were used in this study, with 20 mice in each group. Hyperoxia saline (HS) and hyperoxia rhIGF-1 (HrGF) groups were placed in a closed oxygen chamber for one week and returned to the normal environment on the 15th day. The hyperoxia rhIGF-1 (HrGF) group was intraperitoneally injected with rhIGF-1 (1.5 mg/kg), while mice in high-oxygen saline (HS) group received normal saline. The air group (AG) was untreated. Changes in retinal blood vessel distributions, expression levels of serum IGF-1 and VEGF, and retinal IGF-1 and VEGF were determined.

Results: On day 20, pronounced neo-vascularization was observed, but the distribution was disordered. Serum IGF-1 levels in AG and HrGF were significantly higher than that in HS group, but VEGF level was lower in HS mice (p < 0.05). VEGF level in hyperoxia rhIGF-1 group on days 11 and 15 decreased, relative to control value, while retinal IGF-1 and VEGF in AG and hyperoxia rhIGF-1 mice were elevated, relative to corresponding values in HS mice (p < 0.05).

Conclusion: Early exogenous supplementation of rhIGF-1 exerts a therapeutic effect on ROP. Thus, rhIGF-1 may be a potential drug regimen for ROP in clinics.

Keywords: Oxygen-induced retinopathy, rhIGF-1, Premature infants, IGF-1, VEGF