Waqas Ahmad Shams1,
Gauhar Rehman1,
Samuel Okwudili Onoja2 
,
Abid Ali1,
Khurshaid Khan1,
Sadaf Niaz1
For correspondence:- Samuel Onoja Email: samonreal@yahoo.com
Accepted: 17 September 2019 Published: 31 October 2019
Citation: Shams WA, Rehman G, Onoja SO, Ali A, Khan K, Niaz S. In vitro antidiabetic, anti-inflammatory and antioxidant potential of the ethanol extract of Uromastyx hardwickii skin. Trop J Pharm Res 2019; 18(10):2109-2115 doi: 10.4314/tjpr.v18i10.16
© 2019 The authors.
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Purpose: To evaluate the in vitro antidiabetic, anti-inflammatory and antioxidant potential of the ethanol extract of Uromastyx hardwickii Skin (UHSEE).
Methods: The in vitro effects of UHSEE at various concentrations (10 - 250 µg/mL) on the activities of x572;-amylase, x572;-glucosidase and glucose uptake by yeast cells were used to evaluate its antidiabetic potential. Nitric oxide (NO), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide inhibitory assay were employed to determine its antioxidant effects, while the anti-inflammatory effects were evaluated using human red blood cell (HRBC) membrane stabilization assay.
Results: UHSEE inhibited x572;-amylase and x572;-glucosidase enzymes but increased glucose uptake by yeast cells in a concentration-dependent manner (p < 0.05). It also inhibited NO, DPPH, hydrogen peroxide and HRBC hemolysis in a concentration-dependent manner (p < 0.05).
Conclusion: Uromastyx hardwickii skin exhibits promising good antidiabetic, antioxidant and anti-inflammatory properties in vitro. However, its true potentials in this regard needs to be evaluted in vivo
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