Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Curcumin attenuates migration and invasion of osteosarcoma cells by regulating expression of miR-33b-5p/SIRT6 axis

Hang Lin , Zhengxu Zhao, Yi Hao, Jian He, Yuan Xu

Department of Orthopedics, Zhejiang Hospital, Zhejiang, Hangzhou City, Zhejiang Province 310012, China;

For correspondence:- Hang Lin Email: HangLindfg@163.com Tel:+8657187987373

Accepted: 16 November 2019 Published: 30 December 2019

Citation: Lin H, Zhao Z, Hao Y, He J, Xu Y. Curcumin attenuates migration and invasion of osteosarcoma cells by regulating expression of miR-33b-5p/SIRT6 axis. Trop J Pharm Res 2019; 18(12):2487-2493 doi: 10.4314/tjpr.v18i12.4

© 2019 The authors.
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Abstract

Purpose: To investigate the potential role of curcumin in osteosarcoma (OS) cell migration and invasion, and also to explore the underlying mechanism of action.

Methods: OS cell lines, Saos-2 and U2OS, were treated with various concentrations of curcumin (0, 10, 20, 50 μM) for 48 h. Transwell assay was used to determine the migratory and invasive activities of human OS cells while miR-33b-5p expression levels were measured using quantitative real time polymerase chain reaction (qRT-PCR). expressions of metastasis-related genes, such as matrix metalloproteinases (MMPs), were quantified by qRT-PCR or western blot. The interaction between miR-33b-5p and Sirtuin 6 (SIRT6) was evaluated by luciferase assay.

Results: Curcumin inhibited the migratory and invasive activities of huamn OS cells, and downregulated MMP2 and MMP9 (p < 0.05). Moreover, in curcumin-treated OS cells, miR-33b-5p expression was upregulated while SIRT6 expression was significantly downregulated (p < 0.05). Overexpression of miR-33b-5p downregulated SIRT6 mRNA and protein expression, whereas transfection with miR-33b-5p inhibitor upregulated SIRT6 expression in OS cells (p < 0.05). Furthermore, curcumin suppressed SIRT6 expression by upregulating miR-33b-5p expression in OS cells. Finally, overexpression of SIRT6 or miR-33b-5p inhibitor reversed the suppression of OS cell metastasis induced by curcumin (p < 0.05).

Conclusion: Curcumin inhibits SIRT6 expression via upregulating miR-33b-5p expression, which leads to the inhibition of OS cell migration and invasion. Thus, curcumin is a potential therapeutic agent for the management of OS.

Keywords: Curcumin, MiR-33b-5p, SIRT6, Osteosarcoma