Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of prednisone on IL-17 secretion in maternal-fetal immune rejection cell model, and on IL-23/IL-17 inflammation axis

Xiaoyan Li¹, Yuhua Pu²

¹The People's Hospital of Honghu, Honghu; ²Renmin Hospital of Wuhan University Qianjiang Hospital, Qianjiang Central Hospital affiliated to Yangtze University, Qianjiang Central Hospital, Qianjiang, PR China.

For correspondence:- Yuhua Pu Email: sbu408@163.com

Accepted: 24 November 2019 Published: 30 December 2019

Citation: Li X, Pu Y. Effect of prednisone on IL-17 secretion in maternal-fetal immune rejection cell model, and on IL-23/IL-17 inflammation axis. Trop J Pharm Res 2019; 18(12):2495-2499 doi: 10.4314/tjpr.v18i12.5

© 2019 The authors.
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Abstract

Purpose: To investigate the influence of prednisone on secretion of IL-17 in maternal-fetal immune rejection cell model, and on tIL-23/IL-17 inflammation axis.

Methods: Four groups of Kunming mice were used. Group A was given blank culture solution. Group B was first given prednisone; thereafter, TGF-β, IL-23, and IL-6 were added. Groups C and D were first treated with culture solution containing TGF-β, IL-23, and IL-6. Then, prednisone was added to group C, while blank culture solution was added to group D. The mRNA expressions of IL-23 and IL-17 in mouse spleen lymphocytes were assayed in the four groups using their respective culture supernatants.

Results: The IL-17 concentration was significantly downregulated in group B, relative to the other groups (p < 0.05). expression of IL-17 mRNA in mouse spleen lymphocytes was significantly downregulated in group B, when compared to the other groups, and was also higher in groups A and D than in group C (p < 0.05). After cytokine stimulation, IL-17 and IL-23 were upregulated in groups C and D. The expression level of mRNA was higher in D than in C (p < 0.05).

Conclusion: Prednisone inhibits the proliferation of lymphocytes. During immune imbalance, prednisone regulates immunity by controlling the secretion of IL-17 via downregulation of the expressions of genes for pro-inflammatory factors IL-17 and IL-23.

Keywords: Prednisone, Maternal-fetal immune, Pro-inflammatory factors, Lymphocytes