Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Protective effect of ginkgolide B against isoproterenol-induced chronic heart failure in rats via modulation of Nrf2 and HO-1 signaling pathways

Lu Hui Zhi¹, Wang Bin Ru², Tan Yun¹, Dong Hui¹, Liu Li¹

¹Department of Critical Care Medicine and Emergency; ²Department of Ear-Nose-Throat (ENT), Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan 430074, China.

For correspondence:- Liu Li Email: LizbethTorresqbl@yahoo.com Tel:+862788843447

Accepted: 29 December 2019 Published: 31 January 2020

Citation: Zhi LH, Ru WB, Yun T, Hui D, Li L. Protective effect of ginkgolide B against isoproterenol-induced chronic heart failure in rats via modulation of Nrf2 and HO-1 signaling pathways. Trop J Pharm Res 2020; 19(1):63-69 doi: 10.4314/tjpr.v19i1.10

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To determine the protective effect of ginkgolide B (GB) against isoproterenol (ISO)-induced chronic heart failure in a rat model.

Methods: A total of 32 male Wistar rats were randomly divided into 4 groups. Rats in control group received only saline, while rats in GB alone group were injected with GB at a dose of 20 mg/kg body weight (bwt) intraperitoneally (i.p). Another group of rats was injected with ISO subcutaneously (s.c.) at a dose of 85 mg/kg for 2 days (ISO group). Rats in the GB+ISO group were administered GB at a dose of 20 mg/kg, i.p., for 7 days prior to exposure to ISO s.c. at a dose of 85 mg/kg.

Results: Rats pre-treated with GB for 7 days prior to ISO exposure showed a significant decrease in cardiac infarct size, and marked decreases in the levels of cardiac biomarkers, inflammatory and apoptotic biomarkers, and lipid peroxidation (p < 0.05), but significant improvement in the levels of endogenous antioxidants (p < 0.05). In addition, GB administration resulted in marked increases in the protein expression levels of heme oxygenase-1 (HO-1) and Nrf2 in cardiac tissue (p < 0.05).

Conclusion: These results indicate that pre-treatment of chronic heart failure rats with GB for 7 consecutive days considerably lowered inflammatory and apoptotic markers via upregulation of Nrf2/HO-1 signaling pathway. Thus, GB has cardioprotective potential in humans.

Keywords: Ginkgolide B, Nrf2/HO-1, Inflammatory markers, Apoptotic markers, Antioxidants