Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Bisleuconothine A potentiates the effect of hyperbaric oxygen therapy against traumatic brain injury by enhancing P2X4 protein expressions

Xiangen Meng, Chunyang Zhang, Na Li, Yu Zhang, Danfeng Fan, Chen Yang, Hang Li, Dazhi Guo, Shuyi Pan

Department of Hyperbric Oxygen, The Sixth Medical Center of PLA General Hospital, Beijing, PR China;

For correspondence:- Shuyi Pan Email: ArielleFlorestyl@yahoo.com Tel:+861066939114

Accepted: 24 January 2020 Published: 29 February 2020

Citation: Meng X, Zhang C, Li N, Zhang Y, Fan D, Yang C, et al. Bisleuconothine A potentiates the effect of hyperbaric oxygen therapy against traumatic brain injury by enhancing P2X4 protein expressions. Trop J Pharm Res 2020; 19(2):247-252 doi: 10.4314/tjpr.v19i2.5

© 2020 The authors.
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Abstract

Purpose: To investigate the effect of bisleuconothine A (BA), alone and in combination with hyperbaric oxygen (HO), on traumatic brain injury (TBI) in rats.

Methods: Traumatic brain injury (TBI) was induced by dropping a 200-g weight of steel on the left anterior frontal areas of Sprague-Dawley rats. The synergistic effect of BA and HO was determined by assessing neurological score, as well as parameters of oxidative stress and inflammation, expressions of P2X4 protein and other proteins, and levels of reactive oxygen species (ROS) in the brain tissues of TBI rats.

Results: Neurological function score, levels of inflammatory mediators and oxidative stress parameters were significantly reduced in rats treated with BA alone, and in those treated with a combination of BA and HO, when compared with untreated TBI rats (p < 0.01). Moreover, treatment with BA alone, and BA-HO combination attenuated the altered protein expressions of P2X4, Akt, PI3K and TLR-4 in the TBI rats, and also upregulated the mRNA expression of P2X4 in the brain tissue, when compared with untreated TBI rats (p < 0.01).

Conclusion: These results suggest that BA, when used alone or in combination with HO, reduces neuronal injury through upregulation of the protein expression of P2X4 in rats with traumatic brain injury. Thus, BA may be used clinically with HO therapy for the management of traumatic injury.

Keywords: Bisleuconothine A, Hyperbaric oxygen, Neuronal injury, Oxidative stress, Inflammatory mediators