Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Hypotensive, vaso-relaxant, cardio-depressant and diuretic effect of crude extract of Crotalaria burhia (Fabaceae)

Muhammad Ali Raza, Imran Imran

Department of Pharmacology, Faculty of Pharmacy, Bahauddin Zakariya University, Multan 60800, Pakistan;

For correspondence:- Imran Imran Email: imran.ch@bzu.edu.pk Tel:+923216302819

Accepted: 27 January 2020 Published: 29 February 2020

Citation: Raza MA, Imran I. Hypotensive, vaso-relaxant, cardio-depressant and diuretic effect of crude extract of Crotalaria burhia (Fabaceae). Trop J Pharm Res 2020; 19(2):323-330 doi: 10.4314/tjpr.v19i2.15

© 2020 The authors.
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Abstract

Purpose: To examine 70 % aqueous-methanol crude extract of Crotalaria burhia (Cb.Cr) for its hypotensive and diuretic effects.

Methods: The effect of intravenous administration of Cb.Cr on blood pressure (BP) of normotensive anesthetized rats was studied. In vitro experiments on rabbit isolated aortic and atrial preparations were performed to elucidate the mechanism of action. The diuretic effect was assessed following oral administration of Cb.Cr in rats.

Results: Intravenous administration of Cb.Cr produced 14.70 ± 1.21, 22.00 ± 2.24 and 36.21 ± 2.65 % reduction in mean arterial blood pressure of the rats at doses of 1, 3 and 10 mg/kg, respectively. It was more potent in relaxing potassium (80 mM)- than phenylephrine (1 µM)-induced contractions in isolated aorta of rabbit with half-maximal effective concentration (EC₅₀) values of 0.58 ± 0.03 and 1.58 ± 0.16 mg/mL, respectively, which are similar to verapamil. The extract showed depressant effects on spontaneously beating atrial preparations of rabbit in a dose-dependent manner. Moreover, Cb.Cr also increased urine volume and urinary electrolyte excretion in rats.

Conclusion: Crotalaria burhia crude extract exhibits hypotensive and diuretic effects in rats. The hypotensive activity of the extract possibly involves vasodilator, cardio-depressant, calcium channel blocking and diuretic actions.

Keywords: Khip, Crotalaria burhia, Calcium channel blocker, Antihypertensive, Diuretic