Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effects of ginsenoside Rg3 on bone loss, bone mineral density and osteoclast number in glucocorticoid-induced osteoporosis rats, and the likely mechanism of action

Maoxiu Peng, Gangyi Jiang, Shaoqi He, Chengxuan Tang, Xiaojun Tang

Department of Orthopaedics, The Third Affiliated Hospital of Wenzhou Medical University, Wenzhou, PR China;

For correspondence:- Xiaojun Tang Email: ek046q@163.com

Accepted: 20 January 2020 Published: 30 April 2020

Citation: Peng M, Jiang G, He S, Tang C, Tang X. Effects of ginsenoside Rg3 on bone loss, bone mineral density and osteoclast number in glucocorticoid-induced osteoporosis rats, and the likely mechanism of action. Trop J Pharm Res 2020; 19(4):811-815 doi: 10.4314/tjpr.v19i4.19

© 2020 The authors.
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Abstract

Purpose: To investigate the effect of ginsenoside Rg3 on bone loss, bone mineral density (BMD) and osteoclast number in glucocorticoid-induced osteoporosis (GIOP) rats, and the mechanism of action involved.

Methods: Sixty female Wistar rats were assigned to control, model group, ginsenoside Rg3, and alendronate sodium groups, comprised of 15 rats per group. The osteoporosis rat model was established via intramuscular injection of dexamethasone. Changes in bone mineral content (BMC), BMD trabecular thickness and area, osteoblasts and osteoclasts in femurs and lumbar vertebrae were measured after 3 months of treatment.

Results: There were significantly higher BMC and BMD levels in ginsenoside Rg3 group than in alendronate rats (p < 0.05). The thickness and trabecular area in femur and lumbar vertebrae in the ginsenoside Rg3 group were significantly higher than those in the model group (p < 0.05), but were comparable with those in the alendronate sodium group (p > 0.05). There were marked increases in osteoblasts, and marked decreases in osteoclasts in the ginsenoside Rg3 group, alendronate sodium and control rats, relative to model rats (p < 0.05).

Conclusion: Ginsenoside Rg3 arrests bone loss, and enhances bone density, trabecular thickness and area, bone microstructure, osteoblast activity and population of osteoclasts number in glucocorticoid-induced osteoporotic rats. This provides a new research direction for the clinical treatment of osteoporosis.

Keywords: Ginseng soap, Rg3, Glucocorticoid, Osteoporosis, Bone loss, Bone mineral density, Osteoclast population