Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

A sensitive and rapid HPLC-DAD method for the determination of 3-hydroxy-1,2-dimethyl-4-pyridone and its distribution in rats

Mengdi Chen, Ping Liu , Xuejiao Zhang, Shuling Li, Qiongyao Zhang

Department of Physical and Chemical Inspection, School of Public Health, Shandong University, Jinan, Shandong, China;

For correspondence:- Ping Liu Email: liupingp@sdu.edu.cn Tel:+8653188382554

Accepted: 29 January 2020 Published: 30 April 2020

Citation: Chen M, Liu P, Zhang X, Li S, Zhang Q. A sensitive and rapid HPLC-DAD method for the determination of 3-hydroxy-1,2-dimethyl-4-pyridone and its distribution in rats. Trop J Pharm Res 2020; 19(4):859-864 doi: 10.4314/tjpr.v19i4.26

© 2020 The authors.
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Abstract

Purpose: To establish a sensitive and rapid method for the determination of the tissue distribution of 3-hydroxy-1,2-dimethyl-4-pyridone (L1) in vivo, and its plasma protein binding capacity.

Methods: This study optimized a reverse-phase HPLC method for specific and sensitive determination of L1 as well as its plasma and tissue distributions. The optimized method was used to determine the plasma protein-binding capacity of L1 in Wistar rats.

Results: A rapid, sensitive and simple HPLC-DAD method was established for studying the plasma and tissue distribution of L1. Following TI administration, its liver concentrations peaked at 60 min and 360min, followed 360 min later with peak level in the kidney (second highest). The L1 concentration was significantly lower after 360 min than after 60 min, and values of its mean binding to plasma proteins was 5.2 % at different L1 concentrations.

Conclusion: These results indicate that L1 is a drug with rapid-absorption and rapid-elimination thath is distributed widely in vivo in rats. Moreover, the drug has a weak plasma protein-binding capacity.

Keywords: 3-Hydroxy-1,2-dimethyl-4-pyridone, Distribution, AlzheimerÃ¢â‚¬â„¢s disease, Therapy