Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Fucoxanthin improves functional recovery of orbitopathy in Graves disease by downregulating IL?17 mRNA expression in a mouse model

Lichao Chai¹, Jing Wang² , Yan Wei¹

¹Department of Nuclear Medicine; ²Department of Nephrology, Xingtai People's Hospital, Xigtai 054031, China.

For correspondence:- Jing Wang Email: jacksonhardingzoy@yahoo.com Tel:+863193286014

Accepted: 22 April 2020 Published: 31 May 2020

Citation: Chai L, Wang J, Wei Y. Fucoxanthin improves functional recovery of orbitopathy in Graves disease by downregulating IL?17 mRNA expression in a mouse model. Trop J Pharm Res 2020; 19(5):933-941 doi: 10.4314/tjpr.v19i5.4

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To explore the efficacy of fucoxanthin (FX), a carotenoid, against inflammation via inhibition of IL-17 mRNA expression, and its anti-oxidant activity in Graves’ orbitopathy (GO)-induced mice model.

Methods: The effects of FX on IL-6, IL-8, IL-17, MCP-1, and TNF-α, in orbital fibroblast tissues extracted from GO-induced BALB/c mice was investigated. Anti-oxidative stress markers, 8-hydroxy-2’-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels were quantified in tear samples collected from GO-induced FX treated mice.

Results: FX administration in cultured human orbital fibroblast cells revealed almost complete cell viability and no cell apoptosis. FX resulted in IL-1β induced Beclin-1 and Atg-5 silencing, in cultured human orbital fibroblasts. BALB/c mice immunized with Ad-TSHR289 indicated elevated levels of thyroid peroxidase and thyroglobulin antibodies in the serum sample. FX predominantly downregulated the mRNA expression of IL-17, and also reduced increased 8-OHdG and MDA in the tear secretion of GO-induced mice.

Conclusion: FX may be an effective and useful molecule for the treatment of GO, through its anti-inflammatory and anti-oxidative potential, but it requires further investigation to ascertain its therapeutic effectiveness.

Keywords: Anti-inflammatory, Anti-oxidant, Fucoxanthin, Graves’ disease, Graves’ orbitopathy, IL-17