Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Vitexin alleviates neuropathic pain in a mouse chronic constriction injury model by inactivation of NF-κB

Wentong Xu¹, Xueli Zhu¹ , Gonghao Zhan², Liangyu Sheng³, Yanwei Chen³

¹Department of Pain Rehabilitation, Lanxi People's Hospital, Jinhua City, Zhejiang Province 321100; ²Department of Pain, the Second Affiliated Hospital of Wenzhou Medical University, Wenzhou City, Zhejiang Province 325003; ³Department of Pain, Lanxi People's Hospital, Jinhua City, Zhejiang Province 321100, China.

For correspondence:- Xueli Zhu Email: XueliZhudhj@163.com Tel:+8657988669782

Accepted: 26 April 2020 Published: 31 May 2020

Citation: Xu W, Zhu X, Zhan G, Sheng L, Chen Y. Vitexin alleviates neuropathic pain in a mouse chronic constriction injury model by inactivation of NF-κB. Trop J Pharm Res 2020; 19(5):989-994 doi: 10.4314/tjpr.v19i5.12

© 2020 The authors.
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Abstract

Purpose: To investigate the therapeutic effect of vitexin on neuropathic pain (NP) in a mouse model of chronic constriction injury (CCI).

Methods: The CCI model was established by four chronic ligatures in the sciatic nerve. Vitexin was intraperitoneally administered (10 mg/kg, once daily) for 21 days. Mechanical withdrawal threshold (MWT) and paw withdrawal latency (PWL) were determined before and after the establishment of CCI model. The spinal cords were collected to measure mRNA levels by reverse-transcriptase polymerase chain reaction (RT-PCR) enzyme-linked immunosorbent assay (ELISA). Western blot was used to examine protein expression levels.

Results: Vitexin reversed the CCI-induced reduction in MWT and PWL values, indicating that it lowered mechanical hypersensitivity response and hyperalgesia caused thermal stimulation (p < 0.05). The elevated levels of IL-6, IL-1β, and TNFα observed in CCI-treated mice were also inhibited by vitexin, suggesting that it suppressed pro-inflammatory cytokines. Moreover, vitexin attenuated CCI-induced activation of NF-κB signaling in CCI-treated mice (p < 0.05).

Conclusion: Vitexin alleviates NP by inhibiting pro-inflammatory cytokines and NF-κB signaling in CCI-treated mice. Thus, it is a potential target for NP treatment.

Keywords: Vitexin, Neuropathic pain, Chronic constriction injury, Mechanical hypersensitivity, Hyperalgesia, NF-κB