Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Albiflorin attenuates inflammation and apoptosis by upregulating AMPK-mediated expression of CDX2 in a mouse model of ulcerative colitis

Xiaoping Xu¹, Haiyan Liu², Yuan Pan¹, Zhaohui Liu¹, Dong Chen¹, Lei Zhang¹, Hong Yuang³

¹Department of Anorectal Surgery, Yuhang District First People's Hospital, Hangzhou City, Zhejiang Province 311100; ²Department of Dermatology, Hunan Academy of Traditional Chinese Medicine Affiliated Hospital, Changsha City, Hunan Province 410006; ³Department of Cardiology, Yuhang District First People's Hospital, Hangzhou City, Zhejiang Province 311100, China.

For correspondence:- Hong Yuang Email: HongYuangkl@163.com Tel:+8657189369151

Accepted: 28 April 2020 Published: 31 May 2020

Citation: Xu X, Liu H, Pan Y, Liu Z, Chen D, Zhang L, et al. Albiflorin attenuates inflammation and apoptosis by upregulating AMPK-mediated expression of CDX2 in a mouse model of ulcerative colitis. Trop J Pharm Res 2020; 19(5):995-999 doi: 10.4314/tjpr.v19i5.13

© 2020 The authors.
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Abstract

Purpose: To investigate the mechanism underlying the ameliorative effect of albiflorin (AF) on ulcerative colitis (UC) in dextran sulphate sodium (DSS)-induced mice model.

Method: Female C57BL/6 mice were administered DSS to establish a mice model of UC. After one week, the mice received AF, and the body weight and length of colon were measured. The histopathological features of colon tissues treated with hematoxylin-eosin (H & E) stain were examined by microscopy. expression of inflammatory cytokines and apoptosis-related proteins were determined using enzyme-linked immunosorbent assay (ELISA) and western blotting.

Results: The relative abundance of goblet cells and crypts of mice were significantly reduced in DSS-induced UC mice model; furthermore, focal ulcers and mucosal damage were apparent. Moreover, treatment with DSS decreased body weight and colon length, downregulated Bcl-2 and AMPK pathway-related proteins, increased inflammatory cytokines levels, and upregulated Bax and cleaved caspase-3. In contrast, treatment with AF completely ameliorated DSS-induced effects.

Conclusion: AF treatment attenuated DSS-induced inflammation response and apoptosis via AMPK pathway and modulation of CDX2 expression in UC mice model.

Keywords: Albiflorin, Ulcerative colitis, AMPK, CDX2, Apoptosis