Zhipeng Yao, Wenge Liu, Chenyang Song
Department of Orthopedic, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, China;For correspondence:- Wenge Liu Email:
Accepted: 20 April 2020 Published: 31 May 2020
Citation: Yao Z, Liu W, Song C. Effect of mesenchymal stem cell-incorporated hydroxyapatite-collagen scaffold on tissue repair in acute spinal cord injury, and the mechanism involved. Trop J Pharm Res 2020; 19(5):1099-1103 doi: 10.4314/tjpr.v19i5.28
© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To study the effect of hydroxyapatite-collagen (HC) scaffold with mesenchymal stem cells (MSCs) on tissue repair in acute spinal cord injury (SCI).
Method: Adult female Sprague-Dawley rats weighing 200 - 230 g were randomly divided into two groups implanted either with bone marrow-MSCs (experimental group) or HC scaffold alone (control group). Spinal cord injury was induced using laminectomy, resulting in a 2.0-mm gap at T10 of the spinal cord. The gap was filled in both groups with 2-mm HC scaffold at day 10 of culture. Cellular development, viability, and proliferation inside the scaffold were determined. Angiogenesis was determined by measuring fibronectin (FN) immunofluorescence, von Willebrand factor (vWF), hypoxia-inducible factor 1-alpha (HIF-1α) and vascular endothelial growth factor (VEGF).
Results: HC scaffold strengthened MSCs. Bone marrow MSCs exhibited no statistically significant difference when compared with cells in culture at day 10 (47.03 ± 3.135 %, p > 0.05). Moreover, on days 5 and 10, FN deposition was higher in MSCs with scaffold than in scaffold-free MSCs. The ex
Conclusion: These findings suggest that HC scaffold with MSCs is a potential therapeutic procedure for spinal cord injury.
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