Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of anti-CIRP antibody on inflammatory response, tumor formation and abdominal aortic aneurysm in rats

Yuqing Wang , Lantao Lu, Weiyan Li, Shuntong Gu

Department of General Surgery, Tianjin 5th Central Hospital, Tianjin, PR China;

For correspondence:- Yuqing Wang Email: muvm3w@163.com

Accepted: 28 May 2020 Published: 30 June 2020

Citation: Wang Y, Lu L, Li W, Gu S. Effect of anti-CIRP antibody on inflammatory response, tumor formation and abdominal aortic aneurysm in rats. Trop J Pharm Res 2020; 19(6):1215-1219 doi: 10.4314/tjpr.v19i6.15

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the effect of anti-cold induced RNA binding protein (CIRP) antibody on inflammation, tumor formation and abdominal aortic aneurysm in rats.

Methods: Thirty healthy male Wistar rats were assigned to pseudo-operation, abdominal aortic aneurysm model, and anti-CIRP groups, with 10 in each group. The levels of CIRP, TNF- α, monocyte giant cytokine chemokine-1 (MCP-1), Toll-like receptor 4 (TLR4)) and nuclear factor kappaB (NF- κB) were determined compared among the groups.

Results: At both 2 and 4 weeks, the expression of CIRP protein in the model group was significantly higher than that in the sham operation group (p < 0.05). At these two time-points, tumor formation and maximum diameter were higher in anti-CIRP and model control rats than in pseudo-operation rats. After 4 weeks of treatment, the protein expressions of TNF- α, MCP-1, TLR4 and NF-κB were higher in anti-CIRP and model control rats than in pseudo-operation rats, but were lower than model control values (p < 0.05).

Conclusion: CIRP expression is significantly increased in abdominal aortic aneurysm tissue and serum, and is involved in the onset and progress of abdominal aortic aneurysm. Anti-CIRP antibody therapy effectively suppresses tumorigenesis, and inhibits tumor wall inflammatory reaction via TLR4/NF-κB pathway. This finding provides a clue and new strategy for the clinical management of abdominal aortic aneurysm.

Keywords: CIRP, Abdominal aortic tumor wall, Inflammatory reaction, Protein expression, Tumor body