Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Synergistic hypolipidemic and hypoglycemic effects of mixtures of Lactobacillus nagelii/betanin in a mouse model

Antonio Rivera¹, Elvia Becerra-Martinez², Yesenia Pacheco-Hernandez³, Gerardo Landeta-Cortes⁴, Nemesio Villa-Ruano⁵

¹Centro de investigación en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla. Ciudad Universitaria, C.P. 72570, Puebla; ²Centro de Nanociencias y Micro y Nanotecnologías, Instituto Politécnico Nacional, CP 07738, Ciudad de; ³Centro de Investigación en Biotecnología Aplicada-IPN, Ex-Hacienda San Juan Molino Carretera Estatal Tecuexcomac-Tepetitla Km 1.5, CP 90700, Tlaxcala; ⁴Centro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, CP 72570, Puebla, México; ⁵CONACyT-Centro Universitario de Vinculación y Transferencia de Tecnología, Benemérita Universidad Autónoma de Puebla, CP 72570, Puebla, México.

For correspondence:- Nemesio Villa-Ruano Email: necho82@yahoo.com.mx Tel:+522214246608

Accepted: 17 May 2020 Published: 30 June 2020

Citation: Rivera A, Becerra-Martinez E, Pacheco-Hernandez Y, Landeta-Cortes G, Villa-Ruano N. Synergistic hypolipidemic and hypoglycemic effects of mixtures of Lactobacillus nagelii/betanin in a mouse model. Trop J Pharm Res 2020; 19(6):1269-1276 doi: 10.4314/tjpr.v19i6.23

© 2020 The authors.
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Abstract

Purpose: To determine the nutraceutical effect of mixtures containing Lactobacillus nagelii/betanin on the carbohydrate and lipid metabolism of mice.

Methods: Lactobacillus nagelii and betanin were isolated from the raw materials. These components were subsequently assessed for their anti-hyperglycemic and hypolipidemic activities, and administered individually or combined in normoglycemic and diabetic mice. These animals were subjected to a standard OGTT and given an atherogenic diet.

Results: The combination of L. nagelii (2.0 × 10⁹ CFU/mL) and betanin (30 mg/kg body weight) exerted a significant (p < 0.01) and prolonged reduction of postprandial blood glucose (30 - 120 min). Interestingly, a mixture of L. nagelii (1 ×10⁷ CFU/mouse/day) with betanin (10 mg/kg body weight) administered for 30 days, produced favourable effects (p < 0.01) in the lipid profile of mice previously treated with an atherogenic diet. These mixtures significantly ameliorated (p < 0.01) hypoinsulinemia, hyperleptinemia and increased the levels of adiponectin.

Conclusion: The simultaneous administration of L. nagelii and betanin in mice produced a beneficial change in blood glucose and lipids in mice, indicating a synergistic nutraceutical effect. However, there is a need to develop this therapy further for potential application in humans

Keywords: Lactobacillus nagelii, Betanin, Anti-hyperglycemic, Hypolipidemic