Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Identification, antioxidant and cytotoxic potentials of casticin in Vitex agnus-castus fruit from different geographical regions of Turkey

Gizem Gulsoy Toplan¹ , Esra Eroglu Ozkan¹, Turgut Taskin², Mahmoud Abudayyak³, Afife Mat¹, Gunay Sariyar⁴

¹Department of Pharmacognosy, Faculty of Pharmacy, Istanbul University, 34116, Beyazit; ²Department of Pharmacognosy, Faculty of Pharmacy, Marmara University, 34668, Haydarpasa, Istanbul; ³Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, Turkey; ⁴Faculty of Pharmacy, Cyprus International University, Lefko?a, Cyprus.

For correspondence:- Gizem Toplan Email: eczgizemgulsoy@gmail.com Tel:+902124400000

Accepted: 16 May 2020 Published: 30 June 2020

Citation: Toplan GG, Ozkan EE, Taskin T, Abudayyak M, Mat A, Sariyar G. Identification, antioxidant and cytotoxic potentials of casticin in Vitex agnus-castus fruit from different geographical regions of Turkey. Trop J Pharm Res 2020; 19(6):1277-1284 doi: 10.4314/tjpr.v19i6.24

© 2020 The authors.
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Abstract

Purpose: To evaluate the antioxidant and cytotoxic effects, and casticin contents of Vitex agnus-castus (VAC) fruit extract from five geographical regions of Turkey.

Methods: Casticin determination in VAC fruit extracts was performed using HPLC-DAD method. The method was validated for linearity, sensitivity, selectivity, accuracy and precision. The methanol extracts of the VAC fruits were analyzed for antioxidant effects using 2,2-diphenyl-1-picrylhydrazyl (DPPH^•) radical scavenging capacity, metal-chelation capacity assay, radical cation scavenging capacity (ABTS^•+), and total phenolic content. The cytotoxic potential of the extracts against NRK-52E and HeLa cells were determined using MTT and LDH assays.

Results: The casticin content of the extracts ranged from 0.048 and 0.152 %. Total phenol content was in the range of 36.67 ± 0.7 to 74.20 ± 1.02 mg gallic acid equivalent (GAE)/100 mg of extracts. High-to-moderate antioxidant properties were observed in the extracts. Cytotoxicity data demonstrated that all extracts showed high cytotoxic effects on HeLa cell line when compared to the NRK-52E cell line (p < 0.05).

Conclusion: These results suggest that VAC fruits (Monk’s pepper fruits) from Aegean sea coasts have a potential for use in the preparation of phytopharmaceutical products.

Keywords: Vitex agnus-castus, Casticin, Antioxidant, Cytotoxic activity, Anticancer