Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Zerumbone decreases BACH1 levels by upregulating miR-708 to inhibit breast cancer cell proliferation and invasion

Fuguang Zhao¹, Bo Ma¹, Zhenye Lv¹, Jie Chen¹, Yuanjie Cai¹, Chunjing Xu¹, Yong Cai²

¹Department of Breast Surgery, Zhejiang Hospital, Hangzhou City, Zhejiang Province 310030; ²The First Department of Breast Surgery, Jilin Cancer Hospital, Changchun City, Jilin Province 130012, China.

For correspondence:- Yong Cai Email: ANBU61kl@163.com Tel:+8643185879136

Accepted: 26 June 2020 Published: 31 July 2020

Citation: Zhao F, Ma B, Lv Z, Chen J, Cai Y, Xu C, et al. Zerumbone decreases BACH1 levels by upregulating miR-708 to inhibit breast cancer cell proliferation and invasion. Trop J Pharm Res 2020; 19(7):1411-1416 doi: 10.4314/tjpr.v19i7.11

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To investigate the potential mechanism by which zerumbone suppresses breast cancer (BC) cells.

Methods: Cell viability and Transwell assays were performed to assess the effect of zerumbone on BC cell growth. The downstream target of zerumbone was determined using quantitative polymerase chain reaction assays and immunoblotting. Cell viability assays and immunoblotting were conducted to detect if zerumbone had any effect on BACH1 (BTB domain and CNC homolog 1) expression.

Results: Zerumbone suppressed the proliferation, migration, and invasion of BC cells. It also upregulated the expression of microRNA (miR)-708 and, hence, suppressed BACH1 expression. Furthermore, zerumbone suppressed the proliferation and invasion of BC cells by promoting miR-708 expression and suppressing BACH1.

Conclusion: The findings help clarify the anti-tumor mechanism of zerumbone and provide theoretical and therapeutic bases for the anti-tumor effects of Chinese herbal medicine.

Keywords: Breast cancer, Zerumbone, Cell invasion, MiR-708, BACH1